FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335663
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13410.39950783970827886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTA150.002269843869.6368146
TATAGAT350.00565133155.3032670
ATATAGT306.512758E-445.69846
GTATCAA13900.041.942581
ATTGATA350.001209395440.29863461
CGTTTGG350.001365425639.3164728
GCTTAGA350.001386635739.193411
GTGGTAT2300.037.2763371
GGTATCA5200.036.2727471
TATCAAC16400.035.5277182
TGGGTTA400.002553912834.60890638
TGGTATC2450.033.574332
ATCAACG17450.033.3899463
AACGCAG17600.032.9106336
TCAACGC17850.032.6417124
CAGAGTA17550.032.080510
CAACGCA18200.032.013995
AGAGTAC17500.031.7798211
ATAATTG450.00403618931.52212565
ACGCAGA18450.031.4131457
Produced by FastQC (version 0.11.5)