FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460875
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC82730.5663044408316933No Hit
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG77930.5334474202104903No Hit
GTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCC76070.5207153247197741No Hit
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA72620.4970993411482844No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG64300.4401471720715325No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCT60540.4144091725849234No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46990.321656541456319No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41750.2857876272781723No Hit
GTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC40550.27757337212287153No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39750.27209720201933774No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC39470.27018054248310086No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCAC39200.26833233507315823No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37930.2596389150337982No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG37550.2570377342346197No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA36900.25258834602549846No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC35150.24060922392401812No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33870.23184735175836402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33130.2267818944125952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32920.22534439976041754No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31630.2165140754684692No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG29700.20330281509369383No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA28660.19618379395909985No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28360.19413023017027464No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG27630.18913322495080004No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27350.1872165654145632No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27180.18605287926756225No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGG26930.18434157611020793No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26470.18119277830067596No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26020.17811243261743817No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT25530.1747582784290237No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25360.17359459228202276No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24970.17092495935655No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT24810.16982972533584323No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24020.16442200735860357No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG23480.16072559253871824No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22730.15559168306665525No Hit
GACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGG22480.15388037990930092No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22040.1508684863523573No Hit
GCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT22030.15080003422606314No Hit
GCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT21470.14696671515358944No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21370.14628219389064773No Hit
CTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGACGGCCCC21360.14621374176435356No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT21130.14463934285958757No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20980.14361256096517497No Hit
ATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGC20970.14354410883888083No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20850.14272268332335072No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGTCCGAAGATG20700.14169590142893812No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20530.1405322152819372No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20340.13923162488234792No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20340.13923162488234792No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATC20170.13806793873534698No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20100.13758877385128776No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19940.136493539830581No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19190.13135963035851803No Hit
GTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACC19040.13033284846410542No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18600.1273209549071618No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC18270.1250620347394541No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18170.12437751347651237No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18100.12389834859245315No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17860.12225549756139299No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17660.12088645503550956No Hit
ATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC17560.12020193377256781No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAA17370.11890134337297852No Hit
GGCCATCAGCGGTCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAA16920.11582099768974075No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16290.11150851373320785No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAG16280.11144006160691367No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16210.11096089672285446No Hit
CTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC16190.1108239924702661No Hit
GGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTAT16060.10993411482844186No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCT15900.1088388808077351No Hit
CCCCTGAGGGCCGCTGTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGA15830.10835971592367587No Hit
ACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGGC15770.10794900316591086No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGA15740.10774364678702833No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATG15530.10630615213485069No Hit
GAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATT15380.1052793702404381No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG15310.10480020535637888No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15260.10445794472490802No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT15250.10438949259861385No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15180.10391032771455463No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15160.10377342346196629No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC15150.10370497133567211No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTC14990.10260973731496534No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAG14970.102472833062377No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14730.10082998203131685No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14640.1002139128946693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4150.044.4722481
GGTATCA11900.042.2195661
TGGTATC4350.040.839372
GTATCAA31000.040.572291
TATCAAC34550.036.0919042
GCCAGAG27200.035.76947470
TCAACGC36750.033.8383374
ATCAACG36950.033.655183
CAACGCA37300.033.4309735
AACGCAG38400.032.5633966
AATGTTG34200.032.43099270
CTACGCG450.004688163330.57915733
CGCAGAG41700.029.834838
ACGCAGA42050.029.7399027
AGAGTAC42450.029.2362111
CAGAGTA43000.028.94176910
GCAGAGT43550.028.7332699
GTACTAG605.728991E-428.4814531
ACTGGTG45250.027.23486370
AGTACGG33300.024.84638813
Produced by FastQC (version 0.11.5)