FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057384
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCGCTCAGACTGTTCATTTGCAGATACA20880.19746846935455803No Hit
GTCTATTACTGTGCAAGGGGGCCAGTTTGGGGGGGTTATCGTTTTGACTA16200.15320829518888124No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15820.14961452036346304No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAT15700.14847964410280465No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATGACACTCAGG14900.1409138023650821No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13540.1280518714109538No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12240.11575737858715472No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12200.11537908650026858No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGG11520.10894812102320445No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11370.10752952569738146No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10970.10374660482852019No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10780.1019497174158111No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT10610.10034197604654506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.047.0093961
CTCGTAT1050.042.8640439
GTATCAA19300.042.1541941
TCGTATG1300.039.9567540
TATCAAC21350.037.930782
AATAGAC5250.036.101493
ATCAACG22750.035.8995253
TAATAGA5250.034.7887082
TCAACGC23400.034.7550474
CAACGCA23650.034.533375
AACGCAG23950.033.9569176
CGTATGC1550.033.5264541
ATAGACA5800.033.272224
CGCAGAG26200.031.1722978
AGAGTAC25900.031.00115811
GGTATCA12150.030.9268951
GTAATAG6200.030.581381
CAGAGTA26550.030.37197910
ACGCAGA26950.030.1769247
TCTCGTA1401.4551915E-1129.66101538
Produced by FastQC (version 0.11.5)