FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465628
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT8050.1728847921516747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16250.041.3462451
GGTATCA6400.040.7068021
TGGTATC2050.040.1104472
TATACAC1700.038.2917143
GTGGTAT2250.038.088241
TCGTATG1350.035.94684240
TATCAAC18800.035.901162
AGGGGGG2500.032.83848670
TACACAT2000.032.547965
AACGCAG21050.032.3892676
CAACGCA20950.032.380335
ATCAACG20850.032.371313
TCAACGC21100.031.9877664
CTTATAC2050.031.7711641
CGTATGC1750.031.72983241
TTATACA2100.030.9980532
CTTAGAC450.00477968130.454233
CTCGTAT1600.030.30709839
ACGCAGA22300.030.2664497
AGAGTAC22150.029.69802911
Produced by FastQC (version 0.11.5)