FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688436
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19020.2762784049642959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4400.058.1276870
CTTATAC3050.049.5153051
TATACAC3200.047.1737063
TCGTATG3200.046.55816340
TTATACA3250.045.3923232
CTCGTAT3200.044.34711539
CGTATGC3600.041.41845741
GGTATCA9450.040.679241
GTATCAA23850.040.151561
TGGTATC3900.038.706632
TACACAT4000.038.596675
CTCTCTA3850.037.50335329
ACGAGAC3950.037.3481122
GTGGTAT3950.036.4954571
TCTACAT4000.036.16542432
ATACACA4150.035.5482034
TATCAAC27500.034.6824232
TATGCCG4350.034.33785243
TACATCT4000.033.63123334
GTATGCC4350.033.5146542
Produced by FastQC (version 0.11.5)