FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688436
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34950.5076724633807644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4150.038.001311
GGTATCA11200.037.0387341
GTATCAA26550.035.7687761
ACGGGTA1800.032.37442816
TGGTATC5000.031.5296332
TATCAAC30600.031.02342
ATCAACG33100.028.680243
AACGCAG33500.028.4400966
CAACGCA33700.028.2692575
TCAACGC34650.027.2963984
CGCAGAG35050.026.9927278
CAGAGTA34450.026.9692510
ACGCAGA35250.026.9309527
AGAGTAC34600.026.85233111
GCAGAGT36450.025.8656869
TACTACC750.002070746922.8475572
AGTACGG36750.022.76256813
GAGTACG37250.022.45703312
GCTTACC800.003005920721.4273681
TAACACT1154.8436625E-520.8592994
Produced by FastQC (version 0.11.5)