FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1233194
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCAGTAGTATCCCACAC29910.2425409140816449No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCA29790.2415678311766032No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28950.23475625084131124No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG26400.21407823910917506No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24990.20264451497493502No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22810.1849668422000107No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT22530.18269631542158005No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22470.1822097739690592No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22260.18050687888523623No Hit
CCCCAGGACAGACGGCCAGGTTTACCTGTGGGACAAACAACGTTGGAAGT21850.17718217895967706No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21260.17239785467655536No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20570.16680262797256556No Hit
GTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGAC19510.15820706231136383No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18860.15293619657572127No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18510.15009803810268296No Hit
GGTTTACCTGTGGGACAAACAACGTTGGAAGTAAAAGTGTGCACTGGTACCAACAGAAGCCAGGCCAGGCCC18430.1494493161659885No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18270.14815187229259955No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18020.14612461624042933No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17140.13898867493679015No Hit
CTACTGGTGATCGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA16640.13493416283244972No Hit
CACCAGTAGTATCCCACACCTGACAGTAATAATCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG16410.1330690872644531No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15970.12950111661263353No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG15580.12633859717124798No Hit
GCCCTCAGGGATCCCTGACCGAATCTCTGGCTCCAAGTCTGGGAACACGG15140.12277062651942841No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14720.11936483635178244No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14690.11912156562552201No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14660.11887829489926158No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14500.11758085102587265No Hit
GCACAGGGGCCTGGCCTGGCTTCTGTTGGTACCAGTGCACACTTTTACTT14290.11587795594204967No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14220.115310324247442No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14090.11425615110031351No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14090.11425615110031351No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGATTAT13950.11312088771109818No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13900.11271543650066412No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13640.1106070902064071No Hit
GGATACTACTGGTGATCGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC13530.10971509754345221No Hit
GGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGATTCGGTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA13420.10882310488049732No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGATCACCAG13360.10833656342797646No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGTTTAC13260.10752566100710836No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13100.10622821713371944No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13040.10574167568119858No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12980.10525513422867773No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT12940.10493077326033048No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC12800.10379550987111517No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA12700.10298460745024708No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG12660.10266024648189985No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGACCG12640.10249806599772623No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12420.10071408067181643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)