FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190831
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16040.1346958552473021No Hit
GTACAATAACTGGCCTCCCCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14480.12159575959980887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27800.039.206131
GGTTACG650.001844636236.98679470
TATCAAC29500.036.8151442
ATCAACG31500.034.4776763
TCAACGC31650.034.3142784
CAACGCA31850.034.09885
TCGTATG1650.033.5972340
AACGCAG33300.032.7169046
GAACGTT450.004020156331.5545362
CGTATGC1650.031.54160341
CTCGTAT1800.030.7634939
ACGCAGA35550.030.7412917
TATCTCG1850.029.83460236
CGCAGAG37050.029.404248
AGAGTAC36500.029.37925111
ACCGATC2400.027.1556927
ACGAGAC2300.026.81454322
GCAGAGT40550.026.781789
GTGGTAT4450.025.4170761
AGTACGG24500.024.05202313
Produced by FastQC (version 0.11.5)