FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324776
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG102880.7765841168620204No Hit
GATTATTACTGTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGG93670.7070629298839954No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCA82170.6202558017355386No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC81260.6133867159429217No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCAACTAGTCAGA65150.49178125207582263No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG60970.4602287481053401No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT60330.4553977427127303No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC55570.41946714010519515No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC50750.38308363074210283No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT47760.36051377742350404No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46970.35455050514200137No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG46530.35122918893458216No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTC45930.3467001213790105No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG45180.3410387869345459No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT43290.3267722241344952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA40750.30759917148257515No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC40660.3069198113492394No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG36410.27483891616394013No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC34720.26208204254908No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC34650.2615536513342633No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA33390.2520426094675628No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC32810.24766451083051022No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA32770.24736257299347209No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32750.24721160407495305No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG32620.2462303061045792No Hit
ACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGG32550.2457019148897625No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32440.2448715858379077No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31620.23868186017862641No Hit
CCCCTGAGGGCCGCTGATTAGTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTACCAGTATA31130.2349831216749096No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30960.2336998858674976No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30380.22932178723044497No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT30270.22849145817859018No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA30260.22841597371933067No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29650.22381142170449947No Hit
ATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG29610.22350948386746136No Hit
GTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACC28650.21626297577854672No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT28120.21226229943779173No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG27720.20924292106741066No Hit
GATTAGTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGG27500.20758226296370103No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC27490.20750677850444152No Hit
GGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG27340.2063745116155486No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26650.20116608392664118No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC26590.20071317717108403No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC26110.19708992312662668No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAA25800.19474990488958133No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25280.1908247130080859No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25130.18969244611919298No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25100.1894659927414144No Hit
GTCCAATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC25000.18871114814881912No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24670.18622016099325472No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24430.18440853397102605No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC23880.18025688871175202No Hit
GTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTACCA23540.17769041709692807No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCAA23360.1763316968302566No Hit
CTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC23250.17550136777840178No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG23150.1747465231858065No Hit
ATGTATACTGGTACCAGCAATTCCCAGGAATGGCCCCCAAACTCCTCATC23070.17414264751173028No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22790.17202908265246353No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC22450.16946261103763957No Hit
TTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCC22430.1693116421191205No Hit
ACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCAACTAGTCAGAC22330.16855679752652525No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGAC21280.16063092930427483No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21150.15964963133390098No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21150.15964963133390098No Hit
GTCGTCCAATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCC20720.1564037995857413No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC20690.1561773462079627No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20660.15595089283018412No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG20380.15383732797091737No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20160.15217666986720774No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA19970.1507424651412767No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG19840.14976116717090285No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG19440.14674178880052174No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC19300.14568500637088835No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19220.14508113069681214No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAG19120.14432628610421686No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA18960.14311853475606442No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTC18960.14311853475606442No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA18870.14243917462272868No Hit
GCTTGGTCCCTCCGCCGAACACCCAACTAGTCAGACTGTCGTCCAATGTTGCACAGTAATAATCAGCCTCATCCT18710.14123142327457625No Hit
GACTAGTTGGGTGTTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18640.14070303205975954No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18290.13806107598567607No Hit
AATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG18140.13692880909678315No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18010.1359475111264093No Hit
CTCCTCATCTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATT17930.13534363545233308No Hit
GATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTACCAGTATACAT17770.13413588410418062No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16950.12794615844489937No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG16630.12553065574859448No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT16630.12553065574859448No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16580.12515323345229684No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACAC16490.1244738733189611No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC16430.12402096656340393No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG16360.12349257534858725No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16230.12251127737821338No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT16060.1212280415708014No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG16020.1209261037337633No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG16000.12077513481524424No Hit
GGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC15990.12069965035598472No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC15860.11971835238561085No Hit
GGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15740.11881253887449653No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15740.11881253887449653No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT15670.11828414765967983No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15600.11775575644486314No Hit
GTTTGGGGGCCATTCCTGGGAATTGCTGGTACCAGTATACATAATTAATG15400.11624606725967258No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGACATTAATTATGTATACTGGTACCAGCAATTCCCAGGAATGGCCCC15390.11617058280041306No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15200.11473637807448203No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT15180.11458540915596296No Hit
GTATACTGGTACCAGCAATTCCCAGGAATGGCCCCCAAACTCCTCATCTA15030.11345314226707005No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAACATTG15020.11337765780781053No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15010.11330217334855101No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTAGTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTA14850.11209442200039856No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG14850.11209442200039856No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG14790.11164151524484138No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGG14580.11005634160039131No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC14550.10982988822261273No Hit
CAACTAGTCAGACTGTCGTCCAATGTTGCACAGTAATAATCAGCCTCATC14470.1092260125485365No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC14210.10726341660778878No Hit
CAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTT14090.10635760309667444No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG14000.1056782429633387No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACC13970.10545178958556013No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC13820.10431952269666721No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGT13810.10424403823740767No Hit
GTATACATAATTAATGTCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA13600.1026588645929576No Hit
CCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13510.10197950445962187No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA13400.10114917540776705No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC13260.10009239297813367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2800.056.6149970
TATACAC2450.050.4490243
CGTATGC2400.049.07046541
TCGTATG2400.049.02752340
TCTCGTA2200.048.696838
CTTATAC2600.047.5636371
TTATACA2800.046.595282
TATGCCG2650.044.5140643
GACCCTA2650.044.08696426
GGTATCA8150.043.8350641
GTATCAA21050.043.7349471
CTCGTAT2500.042.8857739
CACGAGA2950.040.73911721
TATCAAC23350.039.11212
ACCCTAA3150.038.19286727
CTAAGAC3200.037.6416230
TCCGAGC3250.036.9757913
GCCGTCT3250.036.3626546
TAAGACA3450.035.93054231
CCCACGA3250.035.9220519
Produced by FastQC (version 0.11.5)