FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035071
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27170.2624940704550702No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGGCAGTCTACAGTCTGAAGACT20450.1975709878839229No Hit
GTCCCAGACCCACCGCCACTGAATCTGGCTGGGATAGAAGTGGCCCTGGT18580.17950459437082095No Hit
CTTCTATCCCAGCCAGATTCAGTGGCGGTGGGTCTGGGACAGAGTTCACT17830.1722587146195768No Hit
GTCTGAAGACTCTGCAGTTTATTACTGTCAGGAGCATGATGGTTGGGCAC17480.16887730406899623No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17130.16549589351841565No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16620.16056869528756965No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAACA15470.14945834633566202No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15390.14868545249552928No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15280.14762272346534683No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14630.14134296101426858No Hit
GTAATACACAGCCGTGTCTTCATCTTTCAGGTTGTTCATTTGCAGATATA14590.14095651409420223No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14260.13776832700365482No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT13950.13477336337314058No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13670.1320682349326761No Hit
GTGTATTACTGTGCGAGGAGAGCACTTTACGGTATGGACGTCTGGGGCCC13140.12694781324179694No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12780.12346979096119975No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGCC12390.11970193349055282No Hit
GAGTTCACTCTCACCATCGGCAGTCTACAGTCTGAAGACTCTGCAGTTTA12350.11931548657048646No Hit
GGATAGAAGTGGCCCTGGTGGAAGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGG11550.11158654816915942No Hit
GTCTTCAGACTGTAGACTGCCGATGGTGAGAGTGAACTCTGTCCCAGACC11070.10694918512836318No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11040.1066593499383134No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10890.10521017398806458No Hit
CACTTATAGTGTCTGGCGACGAAACTAAGTTTTATGCAGACTCGGCGAAG10820.10453389187794845No Hit
GTCTACAGTCTGAAGACTCTGCAGTTTATTACTGTCAGGAGCATGATGGTTGGGCACAGGCGTTCGGCCAAGGGA10790.10424405668789871No Hit
CATCTATGGTGCTTCCACCAGGGCCACTTCTATCCCAGCCAGATTCAGTGGCGGTGGGTCTGGGACAGAGTTCA10510.10153892824743423No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10490.10134570478740104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18500.043.254171
TATCAAC20400.039.36542
TCGTATG1950.039.05990640
CGTATGC2150.037.0746641
ATCAACG21950.036.2729073
TCAACGC21900.036.199024
CAACGCA22300.035.549715
AACGCAG22350.035.4701776
TATACAC2450.035.018933
TATCTCG2000.034.51362636
AGAGTAC22800.034.46740711
TTATACA2450.033.618172
ACGCAGA23600.033.589847
CTTATAC2500.031.5959631
CGCAGAG25300.031.4684568
GAGACAA5800.031.10306470
CAGAGTA25850.030.6661610
TACGGGG12000.029.17076915
CTCGTAT2500.029.05921639
GTGGTAT3200.028.9772761
Produced by FastQC (version 0.11.5)