FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035071
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25970.2509006628530796No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGGCAGTCTACAGTCTGAAGACT20780.20075917497447032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20520.19824726999403908No Hit
GTCTGAAGACTCTGCAGTTTATTACTGTCAGGAGCATGATGGTTGGGCAC18100.1748672313300247No Hit
CTTCTATCCCAGCCAGATTCAGTGGCGGTGGGTCTGGGACAGAGTTCACT17030.16452977621824977No Hit
GTCCCAGACCCACCGCCACTGAATCTGGCTGGGATAGAAGTGGCCCTGGT16120.15573810878674024No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15930.15390248591642505No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAACA15890.1535160389963587No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14970.1446277598348326No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14380.13892766776385387No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14300.13815477392372116No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14020.1354496454832567No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTACCAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT14010.13535303375324012No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13770.133034352232842No Hit
GTAATACACAGCCGTGTCTTCATCTTTCAGGTTGTTCATTTGCAGATATA13260.128107154001996No Hit
GAGTTCACTCTCACCATCGGCAGTCTACAGTCTGAAGACTCTGCAGTTTA13070.12627153113168083No Hit
GTGTATTACTGTGCGAGGAGAGCACTTTACGGTATGGACGTCTGGGGCCC12720.12289012058110023No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGCC12210.11796292235025424No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11950.11545101736982295No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11640.1124560537393087No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11560.111683159899176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18150.042.2515141
TATCAAC20050.037.7262732
GTGGTAT2750.037.347321
TCAACGC21550.034.9414864
ATCAACG21900.034.539353
CAACGCA21950.034.460675
AACGCAG22350.033.9970666
ACGCAGA24250.031.4821267
AGAGTAC24150.031.20228211
TAACACG450.004805792630.42384
CGCAGAG25600.029.8262588
CAGAGTA26200.028.4994210
AGTACGG23850.024.7013613
GAGTACG24200.024.48564512
GCAGAGT33350.022.697469
AAGCGGT3857.4578566E-1121.84272870
GTACGGG26650.021.20642914
GAGACAA5200.021.14802670
TACGGGA9600.021.05045915
GAGTACT7700.020.90681812
Produced by FastQC (version 0.11.5)