FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962473
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGGTGTCGTCATAGGA19500.20260308600864646No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18910.19647304391915407No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC18750.19481065962369853No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18260.18971960771886587No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17330.18005699900153044No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16950.1761088362998235No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16800.17455035102283387No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGG15910.16530333837936234No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15260.1585499021790741No Hit
GTATATTACTGTGCGAGGGCGAGCAGTTGTAGTGGTGGTAGCTGCTACTCATCAAACTGGTTCGACCCCTGGGGC14440.15003018266486437No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13780.1431728474461102No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12960.13465312793190043No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12870.13371803676570668No Hit
GTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCACCATCTCCTG12800.13299074363644486No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12630.13122446032252333No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12170.1264451054730886No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11820.12280863982677954No Hit
GTTGTAGTGGTGGTAGCTGCTACTCATCAAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCT11810.12270474080831359No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11650.12104235651285802No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACACCAATCGG11610.12062676043899413No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11540.11989946730973232No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11150.1158474055895594No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11140.11574350657109342No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10970.11397722325717188No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10920.11345772816484202No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10880.11304213209097813No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC10870.11293823307251218No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10580.10992516153699897No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10250.10649649392762187No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10190.10587309981682602No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10100.10493800865063228No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10010.10400291748443853No Hit
GTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCA9930.10317172533671073No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9840.10223663417051698No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9650.10026255281966351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15400.040.8403631
TTAACGG350.001341980439.46273435
GGTATCA6550.036.729581
TATCAAC17900.035.125452
TGGTATC3050.034.920922
TTTAACG400.002591744334.5118734
TACCGAG601.3798795E-534.361257
ATCAACG18800.033.2611583
TCAACGC19200.032.5682224
CAACGCA19350.032.493315
AACGCAG19350.032.493316
GTGGTAT3750.031.1606771
TAACCGT450.00433969631.06465753
AGAGTAC20500.030.68964611
CAGAGTA20750.030.31989310
CGCAGAG21300.029.5323778
GCAGAGT21650.029.057979
CGATTGT900.00669674528.42411670
TGTACCG500.00789189127.4861455
TCTGGAG11500.027.25007670
Produced by FastQC (version 0.11.5)