FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575609
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18790.32643686947215905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA400.002750000234.09492
GTATCAA24650.033.628321
GGTATCA12300.029.9525971
AACGCAG27950.029.8890046
TATCAAC28200.029.6214562
GTGGTAT6000.028.9957771
CAGAGTA28250.028.27336910
AGAGTAC28550.028.0958311
ACGCAGA29650.027.9501537
CAACGCA29650.027.9428715
ATCAACG29800.027.9166323
TTAACGG756.221729E-527.558435
CGCAGAG29950.027.451998
TCAACGC30250.027.3886324
GATTAGC500.00818001127.283037
TCTATAC500.008190419527.275923
GCAGAGT30200.027.12129
TGGTATC6300.026.5182552
ATAAGAG801.0352292E-425.5711753
TAACCGT851.265448E-424.8260953
Produced by FastQC (version 0.11.5)