FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491005
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC200.007353794351.75483736
CCGATAA200.007362729451.7389535
GTATCAA16300.042.970791
GTGTATA759.1495167E-1041.18462413
TATCAAC19050.036.752682
GGTATCA6250.035.1585351
TATAAGA905.4478733E-934.3205216
TCAACGC20850.033.7443964
CAACGCA20800.033.6605075
AACGCAG21100.033.3445786
ATCAACG21150.032.9412083
GTGGTAT2400.032.90391
ACGCAGA22050.032.0636147
AGAGTAC22600.030.82772311
CGCAGAG22750.030.7753228
CAGAGTA23050.030.37477710
ATAAGAG1052.446177E-829.4175917
GCAGAGT24000.029.1724439
TGTATAA1103.8440703E-828.08042514
GTATAAG1103.8440703E-828.08042515
Produced by FastQC (version 0.11.5)