FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245506
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA108800.8735405529961318No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83020.6665564035821586No Hit
GTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATT75880.6092303047917874No Hit
TCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT71960.5777571525147209No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC59150.4749073870378786No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51860.41637695844098704No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51490.41340627825157006No Hit
GAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT51210.4111581959460653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51030.40971300017824086No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC49890.4005600936486857No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTT46820.3759114769419015No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC46280.3715758896384281No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC45340.3640287561842336No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43580.3498979531210608No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41980.33705176851817653No Hit
CAGTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTAATGTTCTGA40000.32115461507210724No Hit
GAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTAC39410.3164175844997937No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38590.30983391489081546No Hit
TCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA37870.30405313181951754No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37070.2976300395180754No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36610.2939367614447462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36040.2893603081799686No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32880.26398909358927214No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32220.2586900424405824No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30860.24777078552813073No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC30560.2453621259150899No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAACATTAGGAACTATTTAAATTGGTATCAGCAGAAA29820.23942076553625596No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29660.2381361470759675No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29560.23733326053828724No Hit
GTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA29340.23556691015539066No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29170.2342020030413342No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29070.23339911650365391No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28950.2324356526584376No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28020.22496880785801113No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27790.22312216882134653No Hit
CTGTAACTCTGTTGACAGTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTC27420.22015148863192952No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27340.2195091794017853No Hit
GTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT27130.21782311767265675No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26950.21637792190483227No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAACATTAGGAA26650.21396926229179147No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT26590.2134875303691833No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24560.19718893365427387No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTCGTAGAGGTA24510.1967874903854337No Hit
GTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAA24180.19413796481108883No Hit
GTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGA24130.19373652154224869No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCA23960.19237161442819223No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23090.1853865015503739No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC23090.1853865015503739No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC22980.1845033263589256No Hit
GGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCACTTTGCAAACTGGA22700.18225524405342086No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22240.1785619659800916No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21800.17502926521429846No Hit
GTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTA21670.1739855127153141No Hit
TTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGA21230.17045281194952092No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21120.16956963675807263No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21020.16876675022039236No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20940.16812444099024815No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20530.16483260618575904No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC20340.16330712176416654No Hit
GTCGTAGAGGTACTGTAACTCTGTTGACAGTAGTAAGATGCAAAATCTTC20250.1625845238802543No Hit
CCCCTAAACTCCTCATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCA19970.16033644157474955No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19950.1601758642672135No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA19100.15335132869693122No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18960.15222728754417883No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT18730.1503806485075142No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTA18550.14893545273968972No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18530.14877487543215367No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18020.1446801540899843No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18000.14451957678244826No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATT17980.1443589994749122No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17640.1416291852467993No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17630.14154889659303127No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17410.13978254621013467No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17290.13881908236491836No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17260.13857821640361426No Hit
GTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCACTTTGCAAACT16950.13608926813680544No Hit
CAGTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA16640.1336003198699966No Hit
ACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTG16630.13352003121622857No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16520.1326368560247803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16500.13247627871724424No Hit
CTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG16440.13199454679463607No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16310.13095079429565173No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16230.13030848506550752No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16050.12886328929768304No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15990.12838155737507487No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15970.1282209800675388No Hit
ATCTGGGACAGAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATT15970.1282209800675388No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC15580.12508972257058576No Hit
GTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTCGA15460.12412625872536943No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15440.12396568141783339No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCA15400.12364452680276128No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15390.12356423814899327No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15140.12155702180479258No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG15060.12091471257464838No Hit
GTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACT15020.12059355795957627No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14940.11995124872943205No Hit
ATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCA14700.11802432103899942No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14680.11786374373146334No Hit
CACTATCACTTGCCGGGCCAGTCAGAACATTAGGAACTATTTAAATTGGTATCAGCAGAAACCGGGAAAAGCCC13900.11160122873755726No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13660.10967430104712464No Hit
GGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC13620.10935314643205252No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13550.10879112585567632No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13470.10814881662553212No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13370.10734593008785184No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA13130.10541900239741919No Hit
GCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTCGTAGA12950.10397380662959471No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12760.10244832220800222No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG12700.10196659028539407No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12650.1015651470165539No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12560.10084254913264167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)