FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377890
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5730.1516314271348805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1800.055.91697770
GATATTA200.00816615650.39878527
CTTATAC1800.039.0848731
GTATCAA15600.038.8701251
TATCAAC17350.034.9310072
TATACAC1850.034.3885543
TAAGGCG1900.033.6752129
GTCTTAC502.2091907E-433.5013281
CTCTTAT809.156065E-833.5013241
TTATACA2100.033.483592
CTCGTAT1550.033.393339
TACACAT2250.032.7395135
ACGGGAT1750.032.52691716
TCGTATG1750.031.66097540
AACGCAG19150.031.297986
ACTAAGG2050.031.14071527
GACTAAG2050.031.1076726
CTACACA652.8428261E-530.907934
ACACATC2300.030.5719766
CAACGCA19650.030.5015935
Produced by FastQC (version 0.11.5)