FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377890
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13380.35407129058720793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAT300.00235323568.9937470
GCTTAGT400.007351879551.74530470
GTATCAA18000.032.5976221
TGCCATA850.003488510432.46764470
ACTTTTG950.00601092629.04999470
AACGCAG20400.028.5944656
TAACGGC605.6241074E-428.56458336
TATCAAC20550.028.5488832
AGAGTAC20150.027.81774311
ACGCAGA21100.027.6568157
GCAGAGT20950.027.5565539
GGTATCA8900.027.50131
CAGAGTA20550.027.27627610
CGCAGAG21500.027.0041528
CAACGCA21700.026.8814325
ATCAACG21750.026.8196373
TTAACGG659.1144367E-426.299735
TCAACGC22150.026.1839584
CTAAAAC908.60904E-626.0746483
GCTTGTA1557.4578566E-1125.9579221
Produced by FastQC (version 0.11.5)