FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924711
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17800.040.4667661
CGTATGC1550.040.20680241
TCGTATG1600.038.91209440
TATCAAC20000.035.6608432
GGTATCA7900.035.602951
TCAACGC20600.034.6221774
CAACGCA20850.034.2070435
ATCAACG21250.033.7245063
GTGGTAT3600.032.3948251
AACGCAG22100.032.2722556
TTAACGG553.2835867E-431.31940735
CCCTATA1101.1295924E-931.1720642
TATGCCG2100.029.73001543
AGAGTAC24250.029.12663311
CAGAGTA24600.028.71222910
ACGCAGA25100.028.550087
AGGGGGG3250.028.3619470
CGCAGAG25150.028.2206578
TCTCGTA2100.027.95296938
GAGCGAT2300.026.9151332
Produced by FastQC (version 0.11.5)