FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650401
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGG16840.25891719108672956No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGGCTGCTATCCCATGTT16560.25461215465535875No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15940.24507957398589483No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13600.20910176952372458No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13370.2055654895979557No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13070.20095295056434415No Hit
GTCCGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA12820.1971091680363345No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12260.1884990951735929No Hit
AGCTGGTACAGTCCGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGATACA12000.18450156134446288No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11880.18265654573101825No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11750.18065777881645323No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11390.17512273197611933No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10770.16559015130665544No Hit
CGTCCATACCGTACATCCAGTTAGCGGTCGCACAATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCT10670.16405263829545158No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10360.1592863479607196No Hit
ATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATG10250.15759508364839536No Hit
ATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT9890.1520600368080615No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9300.14298871004195873No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9240.14206620223523642No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9160.14083619182627333No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9020.13868367361058795No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8990.1382224197072268No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8660.13314862677025405No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8610.13237987026465212No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCC8610.13237987026465212No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8560.13161111375905019No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG8430.12961234684448517No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8240.12669107212319786No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8210.1262298182198367No Hit
CAATAATACACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTA8170.12561481301535515No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8050.12376979740191052No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7820.12023351747614164No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC7770.11946476097053971No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACACCACTCAGGC7760.11931100966941932No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA7710.1185422531638174No Hit
GGACAGAAGGTCACCATTTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAATGATCTATCCTGGTACCAGCAG7620.11715849145373393No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG7530.11577472974365044No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7470.11485222193692814No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7030.10808716468763116No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC6780.10424338215962153No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6720.10332087435289922No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG6680.10270586914841767No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT6610.10162961004057498No Hit
GAATTATCCATCCACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAGTG6510.10009209702937111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT253.0341998E-669.22339
TATACCG200.00737129851.7256665
TTAATAG404.5774304E-543.77439560
GTATCAA9350.041.3449171
GGTATCA3450.041.0185621
ATAGCGG459.045586E-538.9988763
TAATAGC459.1335074E-538.9348961
TCGTATG459.791202E-538.47840
TATCAAC10150.038.0510642
CAACGCA10900.035.4328775
CCTTTAA501.719748E-434.96492457
TCAACGC11050.034.9518854
ATCAACG11100.034.7944453
AACGCAG11150.034.6384166
TCTCGTA501.8313837E-434.59281538
TATACAC501.8656808E-434.483783
TGGTATC1300.034.483782
AGAGTAC11750.032.86964411
CAGAGTA11750.032.86964410
CGCAGAG11800.032.730378
Produced by FastQC (version 0.11.5)