FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400567
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT13450.3357740402978778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1950.066.2808170
AACCAAT300.003050749264.6237970
CGTTAGG200.00734974751.76022735
GGGTAAC200.007662848651.217021
CGTATTT200.00768938151.1722614
ATACTGT306.6651986E-445.4864586
TATACAC2050.044.931743
TTATACA2100.043.861942
CTTATAC2200.041.9048271
GTATCAA20850.037.8293531
ACTCCTA2550.037.68050431
TCCTATC2400.035.8540633
GGTATCA8850.035.1092151
TATCAAC22650.035.0938572
AAGGGGG2300.034.82006569
CCTATCT2400.034.47234
TTGAAAA3000.034.2518158
CTTGAAA2900.034.1579457
TCTTATA501.9860374E-434.114842
TACACAT2800.034.114845
Produced by FastQC (version 0.11.5)