FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400567
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24050.6003989345103317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATA200.00761606251.2964216
ATTAGAT200.00761982651.29001215
TGTAGGA306.618593E-445.5512852
TTGAGTA504.1038966E-640.996163
GTGGTAT5000.035.5521931
GTATCAA23000.035.3738371
GGTATCA10700.033.226351
TATCAAC25200.032.4010622
TGGTATC5350.031.928472
CTTGTAT553.4457268E-431.0576952
TAAAAGT450.004798835630.42829324
TTGTATC450.004845999630.367523
AAATTAA900.005668767729.4022570
GCAGAGT27350.029.3763489
CAGAGTA26450.029.21258710
AACGCAG27950.028.972286
AGAGTAC26750.028.8849711
TGAGTAC605.735676E-428.4695534
TCAACGC29050.028.1069434
ACGCAGA29000.027.9302587
Produced by FastQC (version 0.11.5)