FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311272
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28930.2206254690102435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28950.040.192091
TATCAAC31550.036.6510162
ATCAACG33900.034.0096933
TCAACGC34250.033.9609264
CAACGCA34950.033.2807355
AACGCAG35900.032.4950646
GGTATCA15700.031.5139711
ACGCAGA39300.029.6917117
AGAGTAC40300.028.54904711
CGCAGAG40800.028.441538
CAGAGTA41100.028.07641410
GCAGAGT42500.027.2318769
GCCTAAT2356.2773324E-926.54694270
AGTACGG30350.025.53488713
GAGTACG31350.024.82927712
GTGGTAT5700.023.9452821
CCTATAC1352.9677722E-722.740213
GTACGGG34400.022.3301114
TACGGGA13200.021.98257815
GCGTAGA5350.020.9894570
Produced by FastQC (version 0.11.5)