FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715345
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAACCCCTCCCTCGAGAGTCGAATCAGCATGTCAGTTGACTTGTC17090.23890570284268428No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11830.1653747492468669No Hit
GTTGTAGACAGTGCTCCCACTGGCAGAGATTCGCCCAATCCACTCCAGTC11520.16104117593608677No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11050.15447091962619436No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10710.14971796825308067No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10480.1465027364418567No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10090.14105082163152044No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8820.12329715032606645No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8140.11379124757983909No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8100.11323207683006102No Hit
GTCTCTGGTGGCTCCATCAACAGTGGGTTTCGCTTCTGGACTTGGTTCCG7560.10568327170805696No Hit
GGAGTGGATTGGGCGAATCTCTGCCAGTGGGAGCACTGTCTACAACCCCT7550.10554347902061244No Hit
GAAATAGACGGCCGTATCTGCGGCGGTCACAGAGGTCAGGTTCAGGAAGAACTGGTTCCTGGACAAGTCAACTGA7390.10330679602150011No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7390.10330679602150011No Hit
GATATATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATATGGCACAGATTTCA7250.10134969839727682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.044.332541
TATCAAC14400.038.552562
TCAACGC15550.035.923154
CAACGCA15600.035.808015
ATCAACG15700.035.799573
AACGCAG16000.034.9128156
ATAGTGC601.3467015E-534.481796
GTGGTAT1950.033.611741
CAGAGTA17600.031.73891810
AGAGTAC17550.031.4363911
TGTTAGA2209.640644E-1131.02276670
GTGTATA1450.030.927681
ACGCAGA18300.030.711147
CGCAGAG18700.030.0563188
GCAGAGT18750.029.9761689
TAGTGTG605.43695E-428.7348235
GGTATCA7400.027.5037731
TACGCCT1705.456968E-1226.3684274
GTATAGA809.563921E-525.8721941
AGTACGG17650.025.00662213
Produced by FastQC (version 0.11.5)