FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865566
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28990.3349253552011054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35150.039.1780321
GGTATCA14750.036.4627571
GTGGTAT5450.036.4563671
TATCAAC41200.033.3321722
ATCAACG41950.032.4913333
TCAACGC42350.032.184454
AACGCAG42600.032.1563576
CAACGCA42750.031.8032025
AGAGTAC42800.030.8236711
ACGCAGA45200.030.3824217
TGGTATC6450.030.2643662
CGCAGAG45400.029.9554988
CAGAGTA44000.029.90514810
GCAGAGT47700.028.4475069
TAAGCGC500.007319416427.91320247
GAGTACG43250.025.27388212
AGTACGG43150.025.0942213
CATACGC700.001392125624.46182
GTCTAGG1151.8047285E-623.830551
ACTTTCG1400.002222157522.56999470
Produced by FastQC (version 0.11.5)