FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583746
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24500.419703090042587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATC200.007596225551.333244
GTATCAA25150.037.0338481
GTGGTAT5000.034.9275471
TATCAAC28300.032.8919682
GGTATCA10950.031.2718641
AACGCAG30100.030.8113146
TGGTATC5650.030.2850992
CAACGCA31150.029.772735
ATCAACG31300.029.7393843
TCAACGC31350.029.6919524
AGAGTAC30500.029.6446611
ACGCAGA31350.029.5903977
CAGAGTA31050.029.45045710
GCAGAGT32650.028.319519
CGCAGAG32800.028.2895578
CTAGACT500.00804299127.377734
GTAGTAT500.00804299127.377733
GAGTACG32250.025.593512
AGTACGG32400.025.26360113
CCTACAC1653.443347E-922.8147743
Produced by FastQC (version 0.11.5)