FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595148
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.039.6617281
GTATCAA18750.038.880871
TACGAAA400.002487453434.799944
GTACGAA400.002499929934.7644243
ATCAACG21600.034.2254833
TCAACGC21550.034.145344
TATCAAC21700.034.067762
CAACGCA22150.033.2204065
CGATTGT700.00316577433.1258670
AACGCAG22450.032.776486
CGCAGAG23200.031.5660348
ACGCAGA23350.031.216017
AGAGTAC23750.030.69026811
CAGAGTA24400.029.872710
AACGCTA550.00560016129.48372569
CGAAAGG605.115578E-429.03448346
ACGCTAA800.006084436628.98512670
ATGTGCG605.529035E-428.6514877
GCAGAGT26300.027.8453249
GAGTACG19400.027.29275712
Produced by FastQC (version 0.11.5)