FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441644
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26870.6084085824781952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC903.6379788E-1241.77403636
GGTATCA7200.041.486771
TTAACGG955.456968E-1239.4978435
TAACCGT1005.456968E-1239.451453
GTATCAA15700.036.555151
GTACCCT1350.033.9037147
CGTGCAA1303.6379788E-1233.4671157
CGCGGTA1253.6379788E-1233.4019443
AACCGTG1204.7293724E-1132.9961554
TATCAAC17400.032.95752
CAACGCA17900.032.378575
TCAACGC17950.032.288384
GTGGTAT2300.032.1030961
ATCAACG18150.031.9434383
GGTACCC1550.031.69866246
ACCCTAA1357.2759576E-1231.49435249
TTTAACG1209.276846E-1131.15061434
AACGCAG18550.031.063416
CCCTAAC1409.094947E-1230.46440350
AGAGTAC19300.030.02986511
Produced by FastQC (version 0.11.5)