FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239870
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAA150.002376705268.8277725
GGGACGA350.00798429150.66997570
GCATTAG405.0580442E-543.026331
GTATCAA7300.040.5508461
GGTATCA3350.039.044791
TATCAAC8400.035.2406122
GTGGTAT1300.034.4210621
ATCAACG8700.034.025423
TCAACGC8900.032.874054
CAACGCA8950.032.6903955
AACGCAG9100.032.1515436
TACACAG902.0242805E-730.59655
ACGCAGA9900.029.5411197
CAGAGTA10100.028.95614410
TAAGGAT605.482568E-428.684224
CGCGCTA605.4959685E-428.6722615
AGTACGG8650.028.63911413
CGCAGAG10250.028.5323948
AGCGTAC500.007291227.92707450
AATAACA850.007420490527.8188170
Produced by FastQC (version 0.11.5)