FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965172
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37370.38718487482023933No Hit
GAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT35290.3656343118117807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31330.3246053553149076No Hit
CTCCTGATCTGTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATT28880.29922127869436743No Hit
GTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCA27390.2837836157700389No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25600.2652376985656442No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25190.26098975104955385No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24980.25881397305350756No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24280.25156137973335324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23750.24607013050523635No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22360.23166855234092992No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20960.21716336570062125No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20800.21550563008458598No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC20670.2141587198965573No Hit
ACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGA19720.20431591467634783No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18040.18690969070797744No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGAT17830.18473391271193113No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17390.18017513976783414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17330.1795534889118209No Hit
ATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC17250.17872462110380327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16090.1667060378875475No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16030.16608438703153428No Hit
TCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAA16010.16587717007952987No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15790.16359778360748137No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15520.16080035475542184No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15410.1596606615193976No Hit
CCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC14750.15282250210325204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14650.15178641734323No Hit
ATGCAGCACAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAGATAGATATTAATGCT14490.15012868172719473No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14430.1495070308711815No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATATCTATCTAAACTGGTATCAGCAGAAA14320.14836733763515725No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14040.14546630030709554No Hit
GGGTGATGTAACTCTGTTGACAGTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT13730.14225443755102718No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13510.13997505107897867No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAATAT13490.13976783412697424No Hit
GTAGTAAGATGCAATATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA13470.13956061717496984No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13340.1382137069869412No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCC13140.13614153746689708No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12970.13438019337485962No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12930.1339657594708508No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACC12450.12899255262274495No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12190.12629873224668764No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12100.1253662559626678No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11590.12008222368655534No Hit
ATCTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATC11330.11738840331049802No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11290.11697396940648921No Hit
CACCATCAGCAGTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTT11070.11469458293444071No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCT10740.11127550322636794No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10650.1103430269423481No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10580.10961776761033266No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10480.10858168285031063No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10290.10661312180626874No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAATCTTGATGGGACCCCA10220.1058878624742533No Hit
GTGGGGTCCCATCAAGATTCAGTGGTAGTGGATCTGGGACAGATTTCACT10190.10557703704624669No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10130.10495538619023347No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10100.10464456076222682No Hit
GTCTGCAACCTGAAGATATTGCATCTTACTACTGTCAACAGAGTTACATCACCCCGTGGACGTTCGGCCAGGGGA9970.10329765057419818No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9970.10329765057419818No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC9930.10288321667018935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)