FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906766
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA35600.3926040455861821No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA31320.3454033344876186No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29280.3229057992911071No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGTTGTCCCACGCCTGACAGTAATAGTCAG28830.31794310770364126No Hit
CCCCTGTGCTGGTCATCAATCAAGATAGCAAGCGGCCCTCAGGGATCCCT27950.3082382885992638No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24070.265448858911781No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23110.25486178352518735No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21660.23887088841001977No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20630.22751183877648698No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19250.2122929179082586No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAACAGCCTTTGGGTGTTCGGCGGAGGGACCAAG19230.21207235383770454No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19160.2113003795907654No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT18850.20788163649717786No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATCTATCC18750.20677881614440768No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC18610.20523486765052948No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18240.2011544323452798No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18030.19883850960446245No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG17690.19508892040504386No Hit
CCCAAAGGCTGTTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATA17350.19133933120562527No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17120.18880284439425388No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16760.18483269112428122No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG16440.18130366599541667No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16070.17722323069016704No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15380.16961377025605284No Hit
GACTATTACTGTCAGGCGTGGGACAACAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG15340.16917264211494476No Hit
GTGCTGGTCATCAATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCG15270.16840066786800564No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG15170.16729784751523546No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAACTGACT14830.16354825831581687No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG14640.16145289964555354No Hit
GTAATACACGGCCGTGTCGTCAGATCGCAGCCTGCTCAGCTCCATGTAGACTGTGCTGATGGACGTGTCCCTGGT14600.16101177150444548No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14390.1586958487636281No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14310.15781359248141197No Hit
GGATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC14280.1574827463755809No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT14080.15527710567004055No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13860.15285090089394618No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13820.15240977275283812No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13820.15240977275283812No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13790.15207892664700706No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAACAGC13690.15097610629423688No Hit
GTCTGGGGCTGAGGTGCAGAAGTCCGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAAACACCTTCATCGG13560.14954243983563564No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13490.14877046558869653No Hit
GGCCAGTCCCCTGTGCTGGTCATCAATCAAGATAGCAAGCGGCCCTCAGG13420.1479984913417574No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13380.14755736320064933No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13210.14568256860094003No Hit
GCACAGGATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCC12910.14237410754262952No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12830.1414918512604134No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12670.1397273386959811No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA12580.13873480037848793No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12570.13862451834321093No Hit
CTATTACTGTCAGGCGTGGGACAACAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC12440.1371908518846097No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12420.13697028781405565No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCAATCAAGATAGCAAGCGGCCC12410.13686000577877866No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12040.132779570473529No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12010.13244872436769795No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGTTGTCCCACGCCTGACAGTAATAGTCAGC11980.1321178782618669No Hit
GCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC11870.1309047758738197No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAA11660.12858885313300233No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11640.1283682890624483No Hit
GTCTACATGGAGCTGAGCAGGCTGCGATCTGACGACACGGCCGTGTATTA11570.12759631481550918No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT11200.12351587951025954No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11020.12153080287527322No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT10770.11877375199334779No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT10750.11855318792279375No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10580.11667839332308447No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG10530.11612698314669936No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10450.11524472686448323No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10420.11491388075865218No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10330.11392134244115901No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10270.11325965022949691No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10140.11182598377089568No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10050.11083344545340253No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10030.11061288138284849No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9950.10973062510063235No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9930.10951006103007833No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9930.10951006103007833No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAA9830.10840724067730816No Hit
GGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAGATA9800.10807639457147711No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9760.10763526643036903No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9620.10609131793649078No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGGCGCAAACTATG9590.10576047183065973No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9550.10531934368955165No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9460.1043268053720585No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9430.10399595926622746No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9280.10234172873707219No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9180.10123890838430202No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9170.10112862634902499No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9130.10068749820791693No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC9110.10046693413736289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)