FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906766
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA33590.3704373564955016No Hit
CCCCTGTGCTGGTCATCAATCAAGATAGCAAGCGGCCCTCAGGGATCCCT28570.31507577478643883No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA28190.31088505744591216No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGTTGTCCCACGCCTGACAGTAATAGTCAG27860.3072457502817706No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27050.2983129054243322No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23140.25519262963101835No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22070.24339245185637748No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19420.21416771250796787No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19270.21251348197881262No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18290.20170584252166493No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC18260.20137499641583387No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18080.19938991978084752No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAACAGCCTTTGGGTGTTCGGCGGAGGGACCAAG18010.19861794553390844No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17790.196191740757814No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17540.19343468987588858No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT17440.19233186952311843No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17300.1907879210292402No Hit
GCACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAGCATATCTATCC16870.18604579351232842No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG16660.18372987077151107No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG16510.1820756402423558No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16410.1809728198895856No Hit
GTAATACACGGCCGTGTCGTCAGATCGCAGCCTGCTCAGCTCCATGTAGACTGTGCTGATGGACGTGTCCCTGGT15980.17623069237267386No Hit
GACTATTACTGTCAGGCGTGGGACAACAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG15280.16851094990328266No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15210.16773897565634355No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15120.16674643733885036No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15110.16663615530357337No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14880.16409966849220195No Hit
GGATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC14770.16288656610415475No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAACTGACT14690.1620043098219386No Hit
CCCAAAGGCTGTTGTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATA14510.1600192331869523No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG14380.15858556672835109No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT14370.15847528469307406No Hit
GTGCTGGTCATCAATCAAGATAGCAAGCGGCCCTCAGGGATCCCTGAGCG13990.1542845673525474No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13730.15141723443534494No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG13510.14899102965925057No Hit
GTCTGGGGCTGAGGTGCAGAAGTCCGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAAACACCTTCATCGG13300.1466751069184332No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13170.14524144045983198No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13070.1441386201070618No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13050.14391805603650776No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13030.14369749196595372No Hit
GCACAGGATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCC13000.14336664586012268No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12970.14303579975429162No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12830.1414918512604134No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAACAGC12730.1403890309076432No Hit
AGCCAGGCCAGTCCCCTGTGCTGGTCATCAATCAAGATAGCAAGCGGCCC12600.13895536444904197No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12530.13818339020210288No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12450.13730113391988674No Hit
CTATTACTGTCAGGCGTGGGACAACAGCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC12360.13630859560239356No Hit
GGCCAGTCCCCTGTGCTGGTCATCAATCAAGATAGCAAGCGGCCCTCAGG12250.13509549321434636No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12240.13498521117906936No Hit
GTCTACATGGAGCTGAGCAGGCTGCGATCTGACGACACGGCCGTGTATTA12140.13388239082629919No Hit
GCCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC12110.13355154472046812No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12030.132669288438252No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT11710.12914026330938744No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11320.12483926393358374No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11280.12439813579247568No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA11280.12439813579247568No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAA11230.12384672561609059No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGGCTGTTGTCCCACGCCTGACAGTAATAGTCAGC11130.12274390526332041No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10920.12042798252250304No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT10880.11998685438139498No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10860.11976629031084093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10730.11833262385223971No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10680.11778121367585465No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGGCGCAAACTATG10450.11524472686448323No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10360.11425218854699008No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10330.11392134244115901No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT10300.11359049633532796No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10280.11336993226477395No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10250.11303908615894287No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAA10220.11270824005311182No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG10200.11248767598255781No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10100.11138485562978762No Hit
GTGCATATAGTAGCCGATGAAGGTGTTTCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCGGACTTCTGCAC9940.10962034306535534No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9480.10454736944261254No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9410.10377539519567341No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9410.10377539519567341No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9360.10322398501928833No Hit
GGACAGACAGCCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAGATA9330.10289313891345728No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9330.10289313891345728No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9230.1017903185606871No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9100.10035665210208589No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9090.10024637006680885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT504.7495505E-664.1855970
GTGGTAT1800.048.01041
CGGAGTA404.6602887E-543.64391356
GTATCAA19400.040.9820671
GTCGGTT551.1367418E-437.51574769
TATCAAC22650.034.9065442
ATCAACG23800.033.1795543
TCAACGC23700.033.1521034
CAACGCA24250.032.398415
AACGCAG24700.032.0871736
TACTATG7300.030.77391870
CGCAGAG26350.029.947158
AGAGTAC26750.029.6281611
CAGAGTA27100.029.24550810
GCAGAGT27250.029.0845229
ACCGGAG605.102879E-429.04887454
GACCGGA605.119564E-429.03267953
TACACTC854.468111E-628.3778655
ACGCAGA28300.027.8836547
GTCTAGC500.00766065827.6539881
Produced by FastQC (version 0.11.5)