FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411947
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC24770.6012909427669093No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCA23700.5753167276372931No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17690.42942417349804707No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15870.38524373281028873No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15170.36825125562268934No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15160.36800850594858076No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAG15030.3648527601851695No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC14290.3468892843011358No Hit
GTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCG14250.34591828560470156No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGG14020.3403350431002046No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13510.32795480972066793No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13370.3245563142831481No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC13010.3158173260152398No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12120.29421260501957774No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12060.2927561069749264No Hit
CCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG11390.2764918788096527No Hit
GTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA11270.27357888272034997No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11250.2730933833721328No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11090.2692093885863958No Hit
GGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAT10700.2597421512961619No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10020.24323517345677964No Hit
GACTATTACTGTCAATCAGCAGACTCCACTGCTTCTTATGTGGTCTTCGG9820.23838017997460836No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9780.23740918127817412No Hit
GGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGG9730.23619543290763134No Hit
CATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCT9700.23546718388530566No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9640.23401068584065426No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9440.22915569235848304No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAG9430.22891294268437443No Hit
TCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAGCA9410.22842744333615733No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9210.22357244985398605No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8990.21823195702359768No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8790.21337696354142643No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8740.21216321517088366No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT8570.20803647071103806No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8240.20002573146545552No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8180.19856923342080413No Hit
CCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCT8170.1983264837466956No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA8070.19589898700560995No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC7950.1929859909163072No Hit
GCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGG7930.19250049156809007No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7480.18157675623320474No Hit
CACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTTTGACCATCG7450.18084850721087906No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7440.18060575753677052No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC7400.17963475884033625No Hit
CTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATA7210.17502251503227356No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7050.17113852024653656No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7020.1704102712242109No Hit
ATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTT6950.16871102350545095No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT6880.16701177578669102No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6870.16676902611258243No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAG6790.16482702871971394No Hit
GTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACA6790.16482702871971394No Hit
GGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCT6790.16482702871971394No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6760.16409877969738826No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6730.16337053067506255No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC6620.16070028425986838No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6530.15851553719289133No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG6450.1565735398000228No Hit
CTCCAGCGATGGTCAAAGTGACTGTTGTTCCTGAACTGGAGCCAGAGAATCGCCCAGGGATCCCTTGAGGCCTCT6380.15487429208126288No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT6330.15366054371072005No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6320.15341779403661152No Hit
GTTCAGGAACAACAGTCACTTTGACCATCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAG6300.15293229468839437No Hit
GCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTT6280.15244679534017724No Hit
CAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGT6210.15074754762141732No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6190.1502620482732002No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6090.14783455153211456No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5920.14370780707226902No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5900.14322230772405187No Hit
GGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCC5890.14297955804994333No Hit
CTCCACTGCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCC5870.1424940587017262No Hit
GCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA5770.14006656196064057No Hit
CCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG5670.13763906521955493No Hit
GCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT5470.13278407173738369No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA5450.13229857238916656No Hit
CACCAATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGA5410.13132757369273232No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC5320.12914282662575524No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5300.12865732727753815No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC5290.12841457760342956No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC5220.12671532988466963No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5180.12574433118823536No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5160.12525883184001826No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC5160.12525883184001826No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT5150.12501608216590968No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5150.12501608216590968No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC5090.12355958412125832No Hit
AGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTT5040.12234583575071552No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT4980.12088933770606414No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4910.11919008998730418No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT4790.11627709389800145No Hit
ATATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCT4660.11312134813459013No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT4640.11263584878637302No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4630.11239309911226444No Hit
GCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAG4610.11190759976404732No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC4590.11142210041583019No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT4580.11117935074172164No Hit
ATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTTTGA4570.11093660106761306No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG4550.11045110171939594No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC4540.11020835204528737No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA4530.10996560237117882No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC4430.10753810563009318No Hit
GCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCA4380.10632435725955038No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT4380.10632435725955038No Hit
CCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGT4360.10583885791133325No Hit
CTATTACTGTCAATCAGCAGACTCCACTGCTTCTTATGTGGTCTTCGGCG4320.104867859214899No Hit
GTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCT4320.104867859214899No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC4300.10438235986668187No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTC4280.10389686051846475No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGG4260.10341136117024763No Hit
GGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGC4250.10316861149613907No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4230.10268311214792195No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC4200.10195486312559625No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTAT4180.10146936377737914No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG4160.10098386442916199No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAAC4150.10074111475505344No Hit
CAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGT4130.10025561540683632No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA4120.10001286573272776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)