FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411947
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC24040.5835702165569843No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCA21230.5153575581324782No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18130.4401051591588238No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15740.3820879870468774No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAG15030.3648527601851695No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14960.3631535124664095No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14250.34591828560470156No Hit
GTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCG13810.3352372999439248No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGG13130.31873032210454255No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC12970.3148463273188056No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12850.3119333312295028No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC12590.3056218397026802No Hit
GGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAT12150.29494085404190346No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11940.28984311088562364No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11480.2786766258766297No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11430.27746287750608695No Hit
CCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG11340.27527813043910987No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10800.2621696480372475No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10510.2551299074880992No Hit
GACTATTACTGTCAATCAGCAGACTCCACTGCTTCTTATGTGGTCTTCGG10170.24687641856840806No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9680.2349816845370885No Hit
GTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA9400.22818469366204874No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9320.22624269626918025No Hit
GGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGG8950.21726095832716344No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8950.21726095832716344No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAG8940.21701820865305488No Hit
CCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCTCAAGGGATCCCT8700.21119221647444936No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8580.20827922038514665No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8530.20706547201460382No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8500.20633722299227814No Hit
TCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAGCA8490.20609447331816957No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8360.20293872755475828No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8280.20099673016188974No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA8260.20051123081367264No Hit
CATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCT8230.19978298179134696No Hit
GCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGG8070.19589898700560995No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT8000.19419973928685No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7990.19395698961274144No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC7690.18667449938948455No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7610.18473250199661606No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7470.1813340065590962No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7410.1798775085144448No Hit
CTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATA7300.17720726209925064No Hit
GTACCAGCAGAGGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACA7250.1759935137287078No Hit
GGCCGGGCCAGGCCCCTGTATTGGTGATATATGAAGACACTGAGAGGCCT7080.17186676926886224No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7010.1701675215501023No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGT7000.16992477187599375No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6930.16822552415723382No Hit
CACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTTTGACCATCG6770.16434152937149682No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6770.16434152937149682No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6740.16361328034917114No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6670.1619140326304112No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC6600.16021478491165125No Hit
GCTTGGTCCCTCCGCCGAAGACCACATAAGAAGCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTT6320.15341779403661152No Hit
ATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGACCTTT6270.1522040456660687No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATAAGAAG6250.15171854631785156No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6110.1483200508803317No Hit
CTCCAGCGATGGTCAAAGTGACTGTTGTTCCTGAACTGGAGCCAGAGAATCGCCCAGGGATCCCTTGAGGCCTCT6080.14759180185800602No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT6070.14734905218389743No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6070.14734905218389743No Hit
CTCCACTGCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCC5990.14540705479102894No Hit
CAGCCACCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGT5950.1444360560945947No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5930.14395055674637755No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAC5930.14395055674637755No Hit
GTTCAGGAACAACAGTCACTTTGACCATCGCTGGAGTCCAGGCAGAAGACGAGTCTGACTATTACTGTCAATCAG5830.14152306000529194No Hit
GGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTCTGCTAATTGGTACCAGCAGAGGCCGGGCCAGGCCC5670.13763906521955493No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5620.13642531684901213No Hit
AGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTT5580.13545431815257788No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC5530.13424056978203505No Hit
GCAGTGGAGTCTGCTGATTGACAGTAATAGTCAGACTCGTCTTCTGCCTGGACTCCAGCGATGGTCAAAGTGA5440.132055822715058No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC5440.132055822715058No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5370.13035657499629807No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG5340.12962832597397236No Hit
GCTTCTTATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT5330.12938557629986383No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5100.12380233379536687No Hit
ACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCT5100.12380233379536687No Hit
CCCTGGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGG5060.12283133509893264No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4980.12088933770606414No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG4970.12064658803195556No Hit
CACCAATACAGGGGCCTGGCCCGGCCTCTGCTGGTACCAATTAGCAGAGA4960.12040383835784702No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC4960.12040383835784702No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC4920.11943283966141276No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA4920.11943283966141276No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC4870.11821909129086994No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC4840.11749084226854425No Hit
TCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCC4820.11700534292032713No Hit
GCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAG4820.11700534292032713No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC4790.11627709389800145No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAAC4710.11433509650513295No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA4610.11190759976404732No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4610.11190759976404732No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG4590.11142210041583019No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT4570.11093660106761306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4560.11069385139350452No Hit
GGACAGACGGCCAGGATCACCTGCTCTGGAGATGCTTTGCCAAAGGTCTC4560.11069385139350452No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA4510.10948010302296168No Hit
AGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCT4490.10899460367474456No Hit
CTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCAGTGTCCCCAGGA4490.10899460367474456No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT4470.10850910432652744No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG4470.10850910432652744No Hit
ATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCTCCAGTTCAGGAACAACAGTCACTTTGA4400.1068098566077675No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC4390.10656710693365895No Hit
GCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTGGGTGTCA4360.10583885791133325No Hit
ATATGAAGACACTGAGAGGCCTCAAGGGATCCCTGGGCGATTCTCTGGCT4330.10511060888900757No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT4310.10462510954079045No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC4300.10438235986668187No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT4260.10341136117024763No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC4210.10219761279970482No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4190.10171211345148769No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG4170.10122661410327057No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT4160.10098386442916199No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA4150.10074111475505344No Hit
CTATTACTGTCAATCAGCAGACTCCACTGCTTCTTATGTGGTCTTCGGCG4140.10049836508094487No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG4120.10001286573272776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAG252.5640408E-455.2052463
CTAAGCA200.00735999951.742354
ACTTGCC200.00736353351.736078
GTATCAA5800.048.3029251
TATCAAC6200.045.1261832
ATCAACG6500.042.4655723
TCAACGC6500.042.455264
GGTATCA1800.040.351831
CAACGCA6850.040.2811285
AACGCAG7000.039.4179536
GTGGTAT655.3332406E-737.247841
CGCAGAG7500.036.7900968
AGAGTAC7650.036.0687211
ACGCAGA7900.034.9273077
GCAGAGT8050.034.276489
CAGAGTA8050.034.2764810
TGGTATC751.6504E-632.2382932
AGTACGG7850.030.75605413
ATAATAC450.00461713530.6695823
GGGCAGT802.702207E-630.263871
Produced by FastQC (version 0.11.5)