FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888712
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.042.2996561
GTATCAA26700.041.601251
CGAATTA557.561208E-637.51278315
TCAACGC30900.035.6128434
ATCAACG31100.035.3838233
CAACGCA31450.034.9900475
TATCAAC31600.034.9327742
AACGCAG32200.034.1750566
ACGCAGA34300.031.9806487
CGCAGAG35000.031.4392818
TGGTATC3950.031.3415552
AGAGTAC34950.031.18909811
CAGAGTA36450.030.18861410
GTGGTAT4450.030.138371
GCAGAGT38600.028.5962099
AGTACGG31400.028.1445413
GAGTACG31700.027.66123812
TACGGGG13350.027.30330815
GTACGGG33800.025.73916814
ACGGGGG6050.025.00992816
Produced by FastQC (version 0.11.5)