FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844592
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20170.23881353363517532No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG19260.22803910053611687No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14960.1771269441339724No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13860.16410290412412146No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13610.16114289503097354No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13540.16031409248489212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12730.1507236630230928No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12660.1498948604770114No Hit
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAAAACTAC12600.14918445829465588No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12310.14575084774660427No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12090.14314603974463408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11730.13888362665050105No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTGAATTGGTATCAGCACAAA11360.13450281319264212No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11260.13331880955538294No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11260.13331880955538294No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11190.13249000700930152No Hit
GTTTTGGCCAGGGGACCAAGTTGGACATCAAACGAACTGTGGCTGCACCA11030.13059560118968686No Hit
GGCCAAAACTGTACGGGAGACTAGAATAATGTTGACAGTAATATGTTGCA10710.1268067895504575No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10660.12621478773182793No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAACTTGGTCCCCTGGCCAAAACTG10400.12313637827495406No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10160.12029476954553205No Hit
GCACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATT10140.1200579688180802No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10130.1199395684543543No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG9950.11780836190728777No Hit
AACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAA9670.1144931517229621No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9590.11354594881315475No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT9570.11330914808570293No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9540.11295394699452517No Hit
GTGTATTATTGTGCAAGACCCCGGCAGGATTATTATTCTTACTTCGGTAT9440.11176994335726599No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGCTGAAAGTAA9390.1111779415386364No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9180.10869153390039214No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9090.10762593062685888No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9070.10738912989940706No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8900.10537632371606645No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8640.10229791425919262No Hit
ATAATACACAGCCGTGTCCTCGGCTCGCAGGCTGTTCATTTGCAGATACA8540.10111391062193342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8530.10099551025820751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16850.040.3624151
TCGTATG350.001319873839.5957440
GTGGTAT2500.038.6659661
GTACTAG459.973868E-538.3590931
TATCAAC19250.035.1487922
TCAACGC20950.032.6261834
ATCAACG20950.032.4614033
CAACGCA21800.031.3540615
AACGCAG22200.030.7891246
AGAGTAC23300.029.03580311
CGCAGAG23550.028.8741348
CAGAGTA23600.028.81296210
ACGCAGA24000.028.3327457
AGTACGG22050.024.88984513
ACGAGAC700.001327886224.66087522
GAGTACG22300.024.61081112
GCAGAGT27850.024.416019
TATACAC1001.5556283E-524.1647973
ATTTCGG750.001899565323.18984448
TTCGGGG6950.022.99491370
Produced by FastQC (version 0.11.5)