FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460743
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16090.34921854482867887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13430.29148570895271336No Hit
GTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAG13080.28388928317955997No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12550.27238612415164204No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCA11250.24417082842278667No Hit
CTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCT10380.22528828435809117No Hit
ATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGA10320.22398603993983632No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10150.22029634742144755No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10120.2196452252123201No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9500.20618869955701985No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9390.20380125145688593No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9010.19555370347460513No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8860.19229809242896798No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8710.18904248138333085No Hit
GTTTAGGAGGCTGCCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACACT8650.18774023696507597No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8350.18122901487380166No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7990.17341554836427248No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7420.1610442263908513No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7380.16017606344534807No Hit
TTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT7370.15995902270897225No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7320.1588738190270932No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7310.1586567782907174No Hit
GTATTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC7260.15757157460883833No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7150.15518412650870442No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA6930.1504092303084366No Hit
GTACCAGCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGA6680.14498321189904134No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6630.14389800821716228No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6490.14085943790790093No Hit
GACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAA6370.13825494907139121No Hit
ATTATAATACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATTAAA6280.13630158244400892No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5990.13000740108911044No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT5880.12761995298897652No Hit
GTGTTGCAGACCCAGGTCTTCACTTCTCTGTTGCTCTGGATCTCTGGTGC5850.12696883077984905No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCGCCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA5370.11655087543381018No Hit
GCAGAGACCAGGGCAGCCTCCTAAACTCCTCATTTACTGGGCTTCTACCCGGGAATCCGGGGTCCCTGACCGATT5360.11633383469743437No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC5350.11611679396105856No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGCTTGGTCCCCTGGCCAAAAGTG5210.11307822365179719No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA5190.11264414217904559No Hit
GTCAGGGACCCCGGATTCCCGGGTAGAAGCCCAGTAAATGAGGAGTTTAGGAGGCTGCCCTGGTCTCTGCTGGTA5110.11090781628803908No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA5060.10982261260616005No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA4930.10700108303327452No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4850.10526475714226803No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA4830.10483067566951641No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4820.1046136349331406No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4780.10374547198763735No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4760.10331139051488573No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4750.10309434977850992No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4720.1024432275693825No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4700.10200914609663088No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4700.10200914609663088No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAACAC4680.10157506462387925No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4670.10135802388750344No Hit
GGCCAAAAGTGTACGGAGTATTATAATATTGCTGACAGTAATAAACTGCC4610.10005577946924858No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA200.007427138351.62462222
GTTATAC200.007433516451.6134153
TCGTATG306.194354E-446.16921640
GTATCAA10600.042.5242581
GGTATCA3900.042.3494721
CGCTAGC350.001233041640.14358559
TTCGCTA350.001245859940.05925857
CTCGTAT350.001327142739.54769539
ATTTCGC350.001345043839.4400835
CGTCTAA459.42628E-538.72387750
ACGTCTA459.4629664E-538.69835349
TATCAAC11800.038.4913642
CTTAGCG451.0163254E-438.232163
TCAACGC12000.037.849844
ATCAACG12250.037.0773933
CAACGCA12600.036.0474625
TGGTATC1151.8189894E-1235.904982
TCGCTAG400.002391292535.07958
AACGCAG13100.034.9342736
GTACGCT400.00246353934.8668251
Produced by FastQC (version 0.11.5)