FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239074
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG29930.24155135205806916No Hit
CTCCTGGACTCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACATTGTCAGGTGTGCAGC29870.24106711947793272No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTGCCC28470.22976835927474873No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGAGCACCACCATAGTAGAGCAGGCAGTAATACTCAG26180.21128681579954062No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA26160.21112540493949516No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC25150.20297415650719813No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC24660.19901959043608372No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT22820.18416979131189906No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGACCTGGGTGCTTG22560.18207145013130774No Hit
CTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC21630.17456584513919265No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAG20280.16367061208612238No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19880.16044239488521267No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19670.15874758085473506No Hit
GAGTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG19480.15721417768430296No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17920.14462413060075507No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16660.1344552464178895No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC16620.1341324246977985No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16420.13251831609734366No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16140.13025856405670685No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15900.12832163373616104No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15680.1265461142756607No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15220.12283366449461454No Hit
GTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC15000.12105814503411419No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC14100.11379465633206733No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13950.1125840748817262No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAACACTGGAGCAGTCACCAGTGGTTA13940.11250336945170344No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13430.10838739252054357No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAG12760.1029801287090198No Hit
CCACCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTG12760.1029801287090198No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12650.10209236897876964No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12460.10055896580833752No Hit
GATTTATAGTACAAGCAACAAACACTCCTGGACTCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC12400.10007473322820107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)