FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239074
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACTCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACATTGTCAGGTGTGCAGC29640.23921089458740966No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA28460.22968765384472598No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG26390.21298162983001823No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC25270.20394262166747104No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTGCCC25260.2038619162374483No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24800.20014946645640214No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGAGCACCACCATAGTAGAGCAGGCAGTAATACTCAG23860.1925631560342643No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT23320.1882050628130362No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGACCTGGGTGCTTG21940.1770677134698977No Hit
CTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC20550.16584965869673646No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19400.156568534244121No Hit
GAGTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG18640.15043492156239258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18320.1478523478016648No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAG17950.1448662468908233No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16670.13453595184791223No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16340.1318726726571617No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16320.13171126179711623No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15940.128644455456252No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15550.12549694368536504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15190.12259154820454629No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14830.11968615272372755No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14110.11387536176209008No Hit
GTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC14100.11379465633206733No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13780.11121208257133958No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAACACTGGAGCAGTCACCAGTGGTTA13530.109194446820771No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAG13520.10911374139074825No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13090.10564340789977031No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGACAATGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC13000.10491705902956562No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC12860.10378718300924723No Hit
GATTTATAGTACAAGCAACAAACACTCCTGGACTCCTGCCCGTTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC12820.10346436128915626No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12510.10096249295845122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA200.00741778151.64626719
GTATCAA31100.043.0528261
GGTATCA11700.042.7673651
TATCAAC35100.037.8118062
ATCAACG35700.036.9533273
GTGGTAT3750.036.8094251
CAACGCA35750.036.4956175
TCAACGC36050.036.1948284
AACGCAG36550.035.7909936
AGAGTAC38900.033.62881511
ACGCAGA39100.033.45687
CGCAGAG39550.033.0761268
CAGAGTA39800.032.9548610
TGGTATC4300.031.2658312
GCAGAGT42450.030.8976089
AGTACGG37600.029.2065613
GAGTACG38650.028.59124612
TACGGGG19900.025.9487115
GTACGGG42550.025.80885114
TACGGGA13500.023.46019415
Produced by FastQC (version 0.11.5)