FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664824
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.042.2120481
GGTATCA7000.039.5027851
ATCAACG20000.035.6885763
TCAACGC19950.035.5971454
CAACGCA20200.035.15135
TATCAAC20700.034.5232772
AACGCAG20650.034.385296
ACGCAGA21650.032.7970547
AGAGTAC22150.032.36794711
GTGGTAT2350.032.3586651
CGCAGAG23000.030.8720118
CAGAGTA23350.030.70449610
GCAGAGT23700.030.2510579
TACGGGC4950.029.9425515
AGTACGG21600.028.72395913
GAGTACG21750.028.6843412
ACGGGTA1555.2750693E-1126.68548416
GTACGGG23800.025.92397314
TGGTATC3000.025.317072
ACGGGCT3350.022.63619416
Produced by FastQC (version 0.11.5)