FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885673
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACGATGTCATCCCATG53250.6012377028542136No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACG51950.586559599310355No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG50540.5706395023897082No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46410.524008296515757No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCC45730.5163305192774309No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT39710.448359609020485No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38740.4374074856069904No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34880.39382480893060984No Hit
GAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGG33570.37903379689795214No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32810.37045275174923475No Hit
GATTATTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGG32240.3640169678876967No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31950.3607426217125282No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCAC31470.35532301425018037No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30370.3429030804822999No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC27910.3151275922377672No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27400.3093692593090226No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26930.30406256033547374No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26450.29864295287312587No Hit
CCCCTGAGGGCCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCAATACA25820.2915297180787943No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA25600.2890457313252182No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25260.2852068427060552No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24120.27233527498297905No Hit
GGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG24040.27143200707258774No Hit
ATGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAG24020.2712061900949899No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACGA23470.26499622321104965No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22450.25347955735356054No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22100.24952776024559856No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG21700.245011420693642No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21630.24422106127204962No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20680.23349475483615284No Hit
CTCCCGGGAGATGCTGGTACCAATACACAAATTGAGTTTCGATGTTGGAGCTGCCTCCAGAACAAGAAATGGTGA20640.23304312088095722No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20540.23191403599296806No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20370.2299945916833865No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19970.22547825213142997No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19950.22525243515383214No Hit
GGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19660.2219780889786637No Hit
ATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCAC19490.22005864466908215No Hit
GCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG19450.2196070107138865No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18680.21091305707637018No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18130.2047030901924299No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17990.20312237134924513No Hit
ATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATG17900.2021061949500549No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17310.19544459411091905No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16400.18516992163021795No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16370.18483119616382118No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA16210.18302466034303858No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16080.1815568499886527No Hit
GTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACC16050.18121812452225597No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCTCTG16000.1806535820782614No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15620.17636305950390269No Hit
GTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15570.17579851705990812No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15540.17545979159351136No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15340.17320162181753312No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC15310.17286289635113636No Hit
GTCAGGGACCCCTGAGGGCCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTA15140.17094345204155484No Hit
GTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCAATACACAAATTGAGTT15100.1704918180863592No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15050.16992727564236462No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT14950.1687981907543755No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14920.16845946528797875No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14890.168120739821582No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14810.16721747191119068No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG14210.1604429625832559No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14150.15976551165046243No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14090.15908806071766893No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAG14080.15897515222887001No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14070.15886224374007113No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14010.15818479280727762No Hit
ACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG13760.1553620805873048No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13680.1544588126769135No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACAC13570.15321681930012546No Hit
CCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGG13550.1529910023225276No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13490.15231355138973413No Hit
CCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGAT13450.15186191743453847No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT13360.15084574103534826No Hit
GCTTGGTCCCTCCGCCGAAAACAACACCATTCACGATGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCT13220.14926502219216348No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG13090.1477972118377776No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCA13010.1468939439273863No Hit
TCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCA12980.14655521846098954No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12600.14226469588663085No Hit
CTCCCGGGAGCGGCCCCCAAACTTCTCATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCT12580.14203887890903302No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12570.14192597042023408No Hit
AAACAACACCATTCACGATGTCATCCCATGCTGCACAGTAATAATCACCC12420.1402323430882504No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG12390.13989361762185368No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12320.13910325820026126No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12250.13831289877866887No Hit
GCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG12150.13718381389067974No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12050.1360547290026906No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12000.13549018655869605No Hit
CTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC11850.13379655922671232No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAG11810.1333449252715167No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG11640.13142548096193515No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATG11620.13119966398433733No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11560.13052221305154385No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11540.13029639607394602No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11440.1291673111859569No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT11440.1291673111859569No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11440.1291673111859569No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11360.12826404327556556No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11240.12690914140997864No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT11140.12578005652198948No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11090.12521551407799494No Hit
GTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGAC11080.125102605589196No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC10980.12397352070120687No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCAC10970.12386061221240796No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT10960.12374770372360906No Hit
CCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCC10700.1208120830148373No Hit
TTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAG10650.12024754057084273No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10610.11979590661564708No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGG10600.11968299812684818No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10520.11877973021645688No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10510.11866682172765794No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG10430.11776355381726664No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10260.11584410950768512No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC10110.11415048217570142No Hit
CAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA10070.11369884822050576No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9970.11256976333251663No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9940.11223103786611988No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC9900.11177940391092424No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGG9780.11042450204533727No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9770.11031159355653836No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9690.10940832564614705No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC9680.10929541715734815No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG9620.10861796622455466No Hit
GGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAGCTCCAACATCGAAACTCAATTTGTGTATTGGTACCAGCAT9480.10703724738136988No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG9440.10658561342617422No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT9350.105569437026984No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9350.105569437026984No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9250.10444035213899487No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG9050.1021821823630166No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG9050.1021821823630166No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8960.10116600596382638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1450.054.7100751
TTACATA200.007365959751.7367022
TTACACC352.275913E-549.2730454
TTATACA751.6370905E-1145.9881822
GGGCAGT1500.045.9881821
GGTATCA5400.044.710731
TGGTATC1800.044.0720062
CTTATAC803.45608E-1143.113921
TATACAC803.45608E-1143.113923
TAGGCAA609.284178E-442.5276670
CGTATGC702.1285814E-839.5524541
TCGTATG702.1338565E-839.54124540
AGACGGA801.6443664E-938.80252525
CGAGACG801.6461854E-938.7959523
GTATCAA12200.038.4491351
AGGGGGG901.5880556E-435.43971670
CTCGTAT806.9465386E-834.5809639
TCTCGTA806.956907E-834.57508538
ATCTCGT806.974187E-834.56530437
ATGGGAC501.8638876E-434.491133
Produced by FastQC (version 0.11.5)