FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885673
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCC54380.6139963620884908No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT48790.5508805168498984No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46910.5296537209557026No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACG42260.47715127366420795No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACGATGTCATCCCATG40330.45535993532601765No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCAC37730.42600372823830013No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37680.42543918579430556No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33900.3827597770283163No Hit
GAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGG33760.38117905818513154No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33490.3781305289875609No Hit
GATTATTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGG33060.3732754639692076No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA30040.33917710035193577No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28940.3267571665840553No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27820.314111415838577No Hit
GGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG27130.30632073011145194No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25790.2911909926123976No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25600.2890457313252182No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25210.2846423002620606No Hit
CCCCTGAGGGCCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCAATACA24960.28181958804208773No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24660.27843233337812034No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG23710.2677060269422236No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23470.26499622321104965No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23130.2611573345918866No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG23020.25991534121509857No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22180.2504310281559899No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21840.2465921395368268No Hit
CTCCCGGGAGATGCTGGTACCAATACACAAATTGAGTTTCGATGTTGGAGCTGCCTCCAGAACAAGAAATGGTGA20490.2313494935489735No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20080.22672024550821807No Hit
GCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG19680.22220390595626152No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19660.2219780889786637No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19650.22186518048986476No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19540.2206231871130767No Hit
GGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19370.2187037428034952No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19350.21847792582589737No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCACGA19060.2152035796507289No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18870.21305831836354955No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18750.21170341649796257No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17840.20142874401726146No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17640.1991705742412832No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16920.19104116304776142No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16830.1900249866485712No Hit
GTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGACC16770.18934753571577773No Hit
ATGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAG16510.18641191500700596No Hit
ACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG16010.1807664905670603No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15980.18042776510066358No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT15900.17952449719027225No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15740.17771796136948964No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCTCTG15480.1747823406607179No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG15460.17455652368312005No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA15390.17376616426152766No Hit
GTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15330.17308871332873418No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15240.17207253692954397No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14950.1687981907543755No Hit
GTCAGGGACCCCTGAGGGCCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTA14910.16834655679917984No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGC14870.1678949228439842No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAG14750.1665400209783972No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14630.16518511911281028No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14620.16507221062401134No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14620.16507221062401134No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAG14590.1647334851576146No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14350.1620236814264407No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14340.16191077293764175No Hit
CCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGG14240.16078168804965265No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14010.15818479280727762No Hit
GTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCAATACACAAATTGAGTT13880.15671698245289176No Hit
CCTCTGAGTATCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGAT13600.15355554476652217No Hit
CTCCCGGGAGCGGCCCCCAAACTTCTCATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCT13580.15332972778892437No Hit
CTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC13480.1522006429009352No Hit
ATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCAC13380.15107155801294608No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13370.15095864952414717No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13100.14791012032657652No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13070.14757139486017978No Hit
GGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAGCTCCAACATCGAAACTCAATTTGTGTATTGGTACCAGCAT13020.14700685241618522No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT12930.145990676016995No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATG12870.14531322508420152No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12750.14395832321861454No Hit
TCCCTGTAGATGAGAAGTTTGGGGGCCGCTCCCGGGAGATGCTGGTACCA12710.1435066892634189No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGG12700.14339378077461998No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12610.14237760437542976No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGATGCCCCCGGGCAGAGGGTCAC12530.14147433646503846No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12320.13910325820026126No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12260.13842580726746778No Hit
GTTCTGGAGGCAGCTCCAACATCGAAACTCAATTTGTGTATTGGTACCAGCATCTCCCGGGAGCGGCCCCCAAA12260.13842580726746778No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG11940.13481273562590257No Hit
GTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAGGGAC11930.13469982713710363No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11900.13436110167070692No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACAC11850.13379655922671232No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT11790.13311910829391885No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11740.1325545658499243No Hit
GGGTCACCATTTCTTGTTCTGGAGGCAGCTCCAACATCGAAACTCAATTT11640.13142548096193515No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG11560.13052221305154385No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11520.1300705790963482No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT11520.1300705790963482No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11490.12973185362995146No Hit
GTACCAGCATCTCCCGGGAGCGGCCCCCAAACTTCTCATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGA11480.12961894514115255No Hit
GCTTGGTCCCTCCGCCGAAAACAACACCATTCACGATGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCT11030.12453806314520145No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10960.12374770372360906No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10830.12227989336922318No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10820.12216698488042427No Hit
ATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATG10750.12137662545883188No Hit
GTGTATTGGTACCAGCATCTCCCGGGAGCGGCCCCCAAACTTCTCATCTA10680.12058626603723949No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10680.12058626603723949No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10490.11844100475006013No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10460.11810227928366339No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10360.11697319439567425No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10330.11663446892927751No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10250.1157312010188862No Hit
AAACAACACCATTCACGATGTCATCCCATGCTGCACAGTAATAATCACCC10210.11527956706369055No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC10070.11369884822050576No Hit
TTACTGTGCAGCATGGGATGACATCGTGAATGGTGTTGTTTTCGGCGGAG10060.11358593973170684No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC10060.11358593973170684No Hit
CCCCCGGGCAGAGGGTCACCATTTCTTGTTCTGGAGGCAGCTCCAACATC10050.11347303124290793No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG10030.11324721426531012No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9960.11245685484371773No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9950.11234394635491879No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT9920.11200522088852206No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC9900.11177940391092424No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG9870.11144067844452749No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCATTCA9850.11121486146692966No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT9840.11110195297813076No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGG9650.1089566916909514No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9630.10873087471335358No Hit
GCCCCCAAACTTCTCATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT9570.1080534237805601No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9560.10794051529176119No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9430.1064727049373753No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9410.10624688795977748No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC9290.10489198609419051No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT9240.10432744365019594No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9220.10410162667259812No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAAC9110.10285963329581009No Hit
CATCTACAGGGATACTCAGAGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC9100.10274672480701115No Hit
ATAATACACAGCCGTGTCTTCGGGTCGCAGACTGCTCATTTGCAGATACA9090.10263381631821225No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG9040.10206927387421769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8980.10139182294142421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)