FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964278
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21120.21902397441401752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16760.17380879787779044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14710.1525493685430965No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14410.14943823254289737No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14330.14860859627617762No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14070.1459122784093384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13140.13626775680872114No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12920.13398625707524178No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11790.12226764480782513No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGGG11420.11843057707424623No Hit
GCCTAGAACCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGCGAC11370.11791205440754637No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11110.11521573654070713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11070.11480091840734725No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9830.10194155627319093No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTGAGCGCGGACCAGTCGCTACGGTG9780.10142303360649108No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGGGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT9750.10111192000647115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.040.29911
GTATCAA22600.039.7195741
TATCAAC26150.034.457172
TCAACGC26450.034.1963774
ATCAACG26500.034.0020753
CAACGCA27200.033.253465
AACGCAG27700.032.653226
TAAGGCG1001.4750185E-830.99405929
TAGCGTT450.004694154530.5701455
CGCAGAG30200.029.9485848
GCCGACA703.619589E-529.8404547
AGAGTAC30350.029.6872611
ACGCAGA30800.029.3651717
TATCGCG854.1258154E-628.67023747
GCAGAGT32300.028.1079339
CAGAGTA32800.027.57461210
GTATAAC756.2940024E-527.5145571
GAGTACG24400.027.20163312
AGTACGG24500.026.9502413
GCCGTCT1305.7698344E-926.76022546
Produced by FastQC (version 0.11.5)