FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964278
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21310.22099436054747695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14220.14746784640943794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13880.14394189227587895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13700.14207521067575948No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13300.13792702934216067No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13010.1349195978753015No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12800.13274180267516214No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12430.12890473494158325No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10970.11376387307394754No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10890.11293423680722779No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10450.10837123734026909No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGGG10440.10826753280692913No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10200.10577862400676984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.040.9587251
GTATCAA25950.040.0326651
TATCAAC27950.036.762542
TCAACGC28700.035.86574
ATCAACG28700.035.7795453
CAACGCA29100.035.4869275
AACGCAG30400.034.082256
CGCAGAG33250.031.0577328
ACGCAGA33750.030.5976187
AGAGTAC33900.030.46223311
GCAGAGT34800.029.7729979
AGTACGG25200.029.2706813
GAGTACG25800.028.72294412
CAGAGTA36150.028.66114410
ATATACG756.356184E-527.4748613
GTACGGG27850.026.2391214
TACGGGG13400.025.09479915
TGTTAGA6850.023.05418670
TACGGGT3150.022.87553215
CAACCCG1202.5792433E-622.8719735
Produced by FastQC (version 0.11.5)