FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927232
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42450.45781422556598567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32980.3556822887907234No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27400.29550317504141355No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26480.28558117062396465No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25200.27177664273881835No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24930.2688647501380453No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24060.25948198509110987No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23920.257972114853672No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA23810.25678578823854226No Hit
CACTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGG22290.240392911374931No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20900.22540205687465487No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20610.22227446852567642No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20460.22065675041413585No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20200.21785270568746548No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA19670.2121367683600221No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18870.20350893843180565No Hit
GTTCTACACACTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGG18860.20340109055770295No Hit
GGTCTAGTCAGAGCCTCCTACATAATAATGGATACAACTATTTGCATTGGTACCTGCAGAAGCCAGGGCAGCCTC18160.19585173937051353No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18080.19498895637769187No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17700.19089073716178906No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG16730.1804294933738266No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16600.17902747101049143No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGACGATGTTGGCGTTTA16480.17773329652125897No Hit
GTGTAGAACTTGCATGCAGTAATAAACGCCAACATCGTCAGCCTCCACTC16100.17363507730535616No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15900.17147811982330202No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15550.16770344422970734No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15470.16684066123688568No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15240.16436016013252347No Hit
CTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC14930.1610168760353396No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTAATCTCCAGTCGTGTCC14910.1608011802871342No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14790.1595070057979017No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14730.15885991855328546No Hit
GTGCAGCCACAGTTCCTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGAG14160.15271258972943125No Hit
GTCAGGGACCCCGGAGGCCCGCTTAGAAGTCAAATAGATCAGGAGTTGTGGAGGCTGCCCTGGCTTCTGCAGGTA13440.14494754279403643No Hit
AGCCAGGGCAGCCTCCACAACTCCTGATCTATTTGACTTCTAAGCGGGCC13420.14473184704583103No Hit
GGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCATCTGTCTTCATC13360.1440847598012148No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13050.14074147570403092No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13010.14031008420762012No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12900.13912375759249032No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12660.1365354086140254No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12610.13599616924351188No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT12400.13373136388735504No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12280.13243718939812257No Hit
TTATTACTGCATGCAAGTTCTACACACTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGG12100.13049592766427387No Hit
GAGATAGTGTGTAGAACTTGCATGCAGTAATAAACGCCAACATCGTCAGC12050.12995668829376034No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11890.12823112230811706No Hit
GAGTGGAGGCTGACGATGTTGGCGTTTATTACTGCATGCAAGTTCTACAC11840.12769188293760356No Hit
GCAGAAGCCAGGGCAGCCTCCACAACTCCTGATCTATTTGACTTCTAAGCGGGCCTCCGGGGTCCCTGACAGGTT11760.1268290999447819No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11730.12650555632247376No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11670.12585846907785753No Hit
CTCCTGATCTATTTGACTTCTAAGCGGGCCTCCGGGGTCCCTGACAGGTT11640.12553492545554942No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11500.12402505521811154No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11490.12391720734400884No Hit
GTAATAAACGCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11470.12370151159580342No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11370.12262303285477637No Hit
GTCGTGTCCCTTGGCCGAAGGAGATAGTGTGTAGAACTTGCATGCAGTAATAAACGCCAACATCGTCAGCCTCCA11330.12219164135836554No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG11270.1215445541137493No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11130.12003468387631142No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11020.11884835726118166No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC10920.11776987852015462No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10700.11539722528989507No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10600.11431874654886803No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10580.11410305080066263No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA10580.11410305080066263No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10490.11313241993373827No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10420.11237748481501932No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10400.11216178906681391No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10110.10903420071783546No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10030.1081714177250138No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10010.1079557219768084No Hit
GGAGATAGTGTGTAGAACTTGCATGCAGTAATAAACGCCAACATCGTCAG9780.10547522087244617No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT9710.10472028575372722No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9690.10450459000552181No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9620.10374965488680286No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9570.10321041551628934No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC9530.10277902401987851No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGACGATGTTGGCGTTTATTACTGCATGCAAGTTCTA9460.10202408890115959No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9440.10180839315295417No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9400.10137700165654334No Hit
CGACTGGAGATTAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT9300.10029852291551627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC609.276846E-1151.99833341
GTGGTAT2550.041.8605961
TCGTATG758.367351E-1041.57157540
GGTATCA7700.040.694391
GTATCAA19700.039.6774251
GTTCGGT350.001311771639.6457642
CGTAGAG801.6552804E-938.77766828
CGAGACG801.6625563E-938.7567423
CGATTGT801.2311539E-437.0081870
TGGTATC2900.035.6191642
TATCAAC22350.034.9710582
ACGAGAC905.2641553E-934.44857822
ATCAACG23150.033.7625583
TCAACGC23750.032.9096074
CAACGCA24050.032.4990925
AACGCAG24400.032.3151446
CGCAGAG25900.030.3074028
AGAGTAC26450.029.67718911
AGACGTA1052.357774E-829.53532225
ACGCAGA26950.029.382097
Produced by FastQC (version 0.11.5)