FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927232
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42390.45716713832136935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29130.31416085726118165No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27360.29507178354500274No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27120.2924834345665378No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26320.2838556046383214No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTA23640.25495237437879625No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23530.25376604776366646No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22110.23845164964108229No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19040.20534235229155165No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19000.2049109607951408No Hit
CACTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGG18950.20437172142462726No Hit
GTTCTACACACTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGG18890.20372463418001102No Hit
GGTCTAGTCAGAGCCTCCTACATAATAATGGATACAACTATTTGCATTGGTACCTGCAGAAGCCAGGGCAGCCTC18760.20232261181667588No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18740.20210691606847045No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18690.20156767669795694No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18690.20156767669795694No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18670.2013519809497515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18480.19930287134180014No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA18220.19649882661512977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17810.19207706377691883No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16820.18140012424075097No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG16360.1764391220320265No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGACGATGTTGGCGTTTA16100.17363507730535616No Hit
GTGTAGAACTTGCATGCAGTAATAAACGCCAACATCGTCAGCCTCCACTC15900.17147811982330202No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15480.1669485091109884No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15090.1627424420209829No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15020.16198750690226393No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14170.15282043760353395No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14010.15109487161789067No Hit
GGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCATCTGTCTTCATC13740.1481829790171176No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT13600.14667310877967973No Hit
CTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC13600.14667310877967973No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTAATCTCCAGTCGTGTCC13310.14354552043070126No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12910.13923160546659305No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12720.13718249585864165No Hit
GTCAGGGACCCCGGAGGCCCGCTTAGAAGTCAAATAGATCAGGAGTTGTGGAGGCTGCCCTGGCTTCTGCAGGTA12700.13696680011043622No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12680.1367511043622308No Hit
GTGCAGCCACAGTTCCTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGAG12530.1351333862506902No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12470.13448629900607398No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12320.1328685808945334No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT12220.13179010215350634No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG12160.1311430149088901No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12160.1311430149088901No Hit
AGCCAGGGCAGCCTCCACAACTCCTGATCTATTTGACTTCTAAGCGGGCC12020.12963314467145223No Hit
TTATTACTGCATGCAAGTTCTACACACTATCTCCTTCGGCCAAGGGACACGACTGGAGATTAAAGGAACTGTGG11980.12920175317504143No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA11960.128986057426836No Hit
GTAATAAACGCCAACATCGTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11880.12812327443401436No Hit
CTCCTGATCTATTTGACTTCTAAGCGGGCCTCCGGGGTCCCTGACAGGTT11800.12726049144119272No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11610.12521138183324132No Hit
GAGTGGAGGCTGACGATGTTGGCGTTTATTACTGCATGCAAGTTCTACAC11490.12391720734400884No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11320.12208379348426285No Hit
GCAGAAGCCAGGGCAGCCTCCACAACTCCTGATCTATTTGACTTCTAAGCGGGCCTCCGGGGTCCCTGACAGGTT10960.11820127001656543No Hit
GAGATAGTGTGTAGAACTTGCATGCAGTAATAAACGCCAACATCGTCAGC10960.11820127001656543No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10860.11712279127553837No Hit
GTCGTGTCCCTTGGCCGAAGGAGATAGTGTGTAGAACTTGCATGCAGTAATAAACGCCAACATCGTCAGCCTCCA10840.11690709552733297No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10590.11421089867476532No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC10550.11377950717835451No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10450.11270102843732743No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10400.11216178906681391No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10370.1118382454445058No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10260.11065191882937604No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10260.11065191882937604No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10220.11022052733296521No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10210.11011267945886252No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10050.10838711347321921No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10020.10806356985091109No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9930.10709293898398674No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9900.10676939536167862No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9850.1062301559911651No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT9590.10342611126449475No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA9560.10310256764218663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.044.949141
GTATCAA21250.042.2870671
TATCAAC23800.037.575092
ATCAACG23900.037.2377553
TCAACGC24400.036.4628834
CAACGCA24600.036.1605875
GTGGTAT2100.036.0686071
AACGCAG25750.034.812416
CGCAGAG26750.033.6394048
AGAGTAC26750.033.5110111
ACGCAGA28000.032.137647
CAGAGTA28200.031.90971810
AGTACGG22200.028.7760213
GAGTACG22350.028.73656712
TGGTATC2800.027.0251582
TACGGGG14100.026.31150615
GTATAGC809.694423E-525.8218461
TGTTAGA10100.025.81573770
GAGTACT10150.025.04012312
AGTACTT10200.024.91737713
Produced by FastQC (version 0.11.5)