FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617535
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12790.20711376683102983No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10040.16258187795023762No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG9840.15934319512254366No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9560.1548090391637721No Hit
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAAAACTAC9310.15076068562915462No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9150.14816973936699943No Hit
GTTTTGGCCAGGGGACCAAGTTGGACATCAAACGAACTGTGGCTGCACCA8840.14314978098407377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8490.13748208603560932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8460.13699628361145522No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8180.13246212765268367No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7990.12938537896637436No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7850.1271183009869886No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7770.12582282785591098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7630.12355574987652522No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7080.11464937210036677No Hit
AACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAA7060.11432550381759737No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAACTTGGTCCCCTGGCCAAAACTG6980.11303003068651979No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTGAATTGGTATCAGCACAAA6960.11270616240375039No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6780.1097913478588258No Hit
GGCCAAAACTGTACGGGAGACTAGAATAATGTTGACAGTAATATGTTGCA6690.10833394058636353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6650.10768620402082474No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6620.10720040159667063No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6510.10541912604143894No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6500.10525719190005425No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGCTGAAAGTAA6450.10444752119313076No Hit
GCACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATT6430.10412365291036137No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT6410.10379978462759194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTT200.00691136952.57493657
CGCGTAT200.007255582651.93273539
ATCGCGT200.00729069251.86937737
TCGCTAG200.007420508751.638388
GTGGTAT1250.046.837761
TCGTATG651.0972144E-842.63230540
TATACAC1150.041.91623
GTATCAA11700.040.6209871
GAGCGAT901.2187229E-1038.35624732
ATCAACG12550.037.3118553
TCAACGC12850.036.440764
TATCAAC12900.036.2995222
CAACGCA13000.036.0202945
AACGCAG13250.035.3406646
CGTATGC904.951289E-934.66134341
AGCGATC1056.348273E-1032.88746333
CGCAGAG14650.032.193218
TTAACGG652.2949795E-531.8962735
CTTATAC1207.8216544E-1131.5695671
AGAGTAC14950.031.31692311
Produced by FastQC (version 0.11.5)