FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617535
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13060.2114859886484167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10390.1682495728987021No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG10280.1664682973434704No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10240.1658205607779316No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9000.14574072724622897No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8560.1386156250253022No Hit
GTTTTGGCCAGGGGACCAAGTTGGACATCAAACGAACTGTGGCTGCACCA8400.13602467876314703No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8160.13213825936991427No Hit
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAAAACTAC8030.13003311553191318No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7790.1261466961386804No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7780.1259847619972957No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7740.12533702543175693No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7650.12387961815929462No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7610.12323188159375582No Hit
GGCCAAAACTGTACGGGAGACTAGAATAATGTTGACAGTAATATGTTGCA7340.11885965977636896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7270.11772612078667606No Hit
AACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATTTGGAA7240.11724031836252197No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTATTTGAATTGGTATCAGCACAAA6540.10590492846559305No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAACTTGGTCCCCTGGCCAAAACTG6520.10558106018282365No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6500.10525719190005425No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6340.10266624563789906No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6330.10250431149651437No Hit
GCACAAACCAGGGCAAGCCCCTAAGCTCCTGATCTACGATGTATCCAATT6300.10201850907236028No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGCTGAAAGTAA6200.10039916765851328No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6190.10023723351712857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA306.0404226E-446.4080548
CCGTTAA350.00136228139.34017616
GTGGTAT2200.037.6465031
GTATCAA12050.036.0844081
TATCAAC13450.032.0250322
CAACGCA13800.031.6736875
TCAACGC13850.031.5593414
TAACGGC553.226999E-431.41034336
GTGTAAC553.250147E-431.3720861
ATCAACG13750.031.3034633
AACGCAG14300.031.045066
AGAGTAC15050.029.95529611
CGCAGAG15200.029.4332778
CAGAGTA15800.028.53336710
CAGTAGT4350.027.57300470
ACGCAGA16300.027.235857
CGTGCAA1056.650298E-726.720257
CTAGTGC700.001116359725.40445366
AGTACGG15050.025.3819713
GAGTACG15200.025.357912
Produced by FastQC (version 0.11.5)