FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991156
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27250.042.4719771
GGTATCA10550.038.199981
TATCAAC30700.037.248392
CAACGCA32150.035.568455
TCAACGC32400.035.5066154
AACGCAG32900.034.862316
ATCAACG33250.034.5989273
CTCGTAT652.2636183E-531.96337339
ACGCAGA35800.031.9388317
AGAGTAC36250.031.5407611
CGCAGAG37650.030.3679288
CAGAGTA38050.030.32021110
GCAGAGT39250.029.3932259
AGTACGG31300.028.16965513
GTGGTAT4200.027.8842751
GAGTACG31750.027.76899712
GTACGGG33950.025.36343414
TACGGGT4550.024.2240315
TACGGGG14100.023.69520415
TACGGGA11900.022.86588115
Produced by FastQC (version 0.11.5)