FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120103
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCAACGGTCACAGAGTTCAGATTCAGGGAGAACTGGTTGTTGGACGTGTCTGCCGA41690.3721979139418429No Hit
GTCTCTGGTGGCTCCATCAGTGGTTACTATTGGGGCTGGATCCGGCAGCC35020.3126498188113057No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34480.30782883359833874No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30420.2715821669971422No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28670.25595860380697133No Hit
GTATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGG25870.2309609027026979No Hit
GTATATTACTGTGCGAGACTCGATAGTGGAAACGATCGACACGGCTATTATTACTACTACCACGGTTTGGACGT24070.2148909519928078No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23770.2122126268744928No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23240.2074809191654696No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21840.1949820686133329No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21250.18971469588064668No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21180.18908975335303985No Hit
GTCCTGGACCTCCTGTGCAAGAGCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC21010.18757203578599468No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20600.1839116581242975No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20500.18301888308485917No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19520.17426968769836346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19280.17212702760371143No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18290.16328855471327192No Hit
GTGTTAGTAGCATTCACCTGGCCTGGTACCAGCAGAGACCTGGCCATGCTCCCAGGCTCCTCATTCATAGTGCAT18120.16177083714622673No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17840.1592710670357994No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG17650.15757479446086656No Hit
GTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA17500.15623563190170903No Hit
ATATTACTGTCAGCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA16850.15043259414535984No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16690.1490041540822585No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16580.14802210153887632No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16470.14704004899549417No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16300.145522331428449No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16110.14382605885351615No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15590.1391836286484368No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15370.13721952356167247No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15190.13561252849068345No Hit
CTATTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGTTTGTCTATTACAATGGGAGAAC15010.13400553341969443No Hit
GTGCAAGAGCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTC14670.1309700982856041No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14480.12927382571067125No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14410.12864888318306442No Hit
GCATTATGGTGGTTCACCTCCTCTCACTTTCGGCGGAGGGACCAGGGTGG14350.1281132181594014No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14290.1275775531357384No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGAGGAGGTGAACCACCATAATG14080.1257027255529179No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCATTCACCTGGCCTGGTACC14000.12498850552136723No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13980.12480995051347957No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGC13790.12311367793854673No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13720.1224887354109399No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13720.1224887354109399No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG13580.12123885035572621No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13420.11981041029262486No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13070.1166856976545907No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT12650.11293604248894967No Hit
CCATAATGCTGACAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCT12620.11266820997711817No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12610.11257893247317433No Hit
CTCCCAGGCTCCTCATTCATAGTGCATCCCGCAGGGCCACTGGCATCCCA12600.11248965496923051No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA12370.11043627237852234No Hit
CAGTAATATACCGCAAAGTCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCA12310.10990060735485932No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC12280.10963277484302783No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11940.10659733970893748No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA11790.10525817714977997No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11620.1037404595827348No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA11550.10311551705512796No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC11400.10177635449597046No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11350.10132996697625128No Hit
CACGTGCACTGTCTCTGGTGGCTCCATCAGTGGTTACTATTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGA11350.10132996697625128No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11310.10097285696047595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)