FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488104
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGACCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCAAAACTAC8630.17680658220379264No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG8070.1653336174257945No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7850.16082638126300952No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6980.14300231098290528No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6870.1407486929015128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6600.13521708488354942No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6430.13173422057594283No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6410.13132447183387147No Hit
GTTTTGGCCAGGGGACCAAGTTGGACATCAAACGAACTGTGGCTGCACCA6220.12743185878419352No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC6180.1266123613000508No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG5640.11554914526412405No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT5620.11513939652205267No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5440.11145165784341043No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5210.10673954730958976No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG5190.1063297985675184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG354.0472514E-969.23048440
CTCGTAT353.2198113E-759.30993739
GTGGTAT1500.046.0118141
CGTATGC602.814977E-740.41351341
TATACAC602.900142E-740.2603383
GTATCAA10200.037.8920821
TCCTAAC501.651354E-435.2048864
TATCAAC10950.034.9815862
GTGTATA709.701489E-734.5088613
ATCAACG12000.032.208273
CTTATAC751.6632912E-632.208271
TCAACGC11900.031.8989474
CTATATT450.004614312730.6745452
CAACGCA12400.030.61275
AACGCAG12800.029.6560526
ACGAGAC703.8397913E-529.57902522
CTCTCTA953.1645868E-729.08690829
GCGTAGG850.006441568528.64648470
AGAGTAC13450.028.47943111
CGTCAGA854.4124663E-628.4190645
Produced by FastQC (version 0.11.5)